Enhancement of the biological autoluminescence by mito-liposomal gold nanoparticle nanocarriers.

Autor: Sardarabadi H; Department of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Chafai DE; Institute of Photonics and Electronics of the Czech Academy of Sciences, Prague, Czechia., Gheybi F; Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Sasanpour P; Department of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Rafii-Tabar H; Department of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: rafii-tabar@nano.ipm.ac.ir., Cifra M; Institute of Photonics and Electronics of the Czech Academy of Sciences, Prague, Czechia. Electronic address: cifra@ufe.cz.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2020 Mar; Vol. 204, pp. 111812. Date of Electronic Publication: 2020 Feb 12.
DOI: 10.1016/j.jphotobiol.2020.111812
Abstrakt: One of the most important barriers to the detection of the biological autoluminescence (BAL) from biosystems using a non-invasive monitoring approach, in both the in vivo and the in vitro applications, is its very low signal intensity (< 1000 photons/s/cm 2 ). Experimental studies have revealed that the formation of electron excited species, as a result of reactions of biomolecules with reactive oxygen species (ROS), is the principal biochemical source of the BAL which occurs during the cell metabolism. Mitochondria, as the most important organelles involved in oxidative metabolism, are considered to be the main intracellular BAL source. Hence, in order to achieve the BAL enhancement via affecting the mitochondria, we prepared a novel mitochondrial-liposomal nanocarrier with two attractive features including the intra-liposomal gold nanoparticle synthesizing ability and the mitochondria penetration capability. The results indicate that these nanocarriers (with the average size of 131.1 ± 20.1 nm) are not only able to synthesize the gold nanoparticles within them (with the average size of 15 nm) and penetrate into the U2OS cell mitochondria, but they are also able to amplify the BAL signals. Our results open new possibilities for the use of biological autoluminescence as a non-invasive and label-free monitoring method in nanomedicine and biotechnology.
Competing Interests: Declaration of Competing Interest We would like to declare that no conflict of interest, financial or other, exists for this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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