Flexible platinum electrodes as electrochemical sensor and immunosensor for Parkinson's disease biomarkers.

Autor: Oliveira GCM; Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, 13600-970, Araras, SP, Brazil., Carvalho JHS; Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, 13600-970, Araras, SP, Brazil., Brazaca LC; Bioanalysis, Microfluidics and Separation Group, São Carlos Institute of Chemistry, University of São Paulo, 13566-590, São Carlos, SP, Brazil., Vieira NCS; Institute of Science and Technology, Federal University of São Paulo, 12231-280, São José dos Campos, SP, Brazil., Janegitz BC; Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, 13600-970, Araras, SP, Brazil. Electronic address: brunocj@ufscar.br.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2020 Mar 15; Vol. 152, pp. 112016. Date of Electronic Publication: 2020 Jan 14.
DOI: 10.1016/j.bios.2020.112016
Abstrakt: In this study, platinum electrodes were fabricated on the bio-based poly(ethylene terephthalate) (Bio-PET) substrates for the development of flexible electrochemical sensors for the detection of Parkinson's disease biomarkers. Dopamine was detected by voltammetric measurements, displaying a 3.5 × 10 -5  mol L -1 to 8.0 × 10 -4  mol L -1 linear range with a limit of detection of 5.1 × 10 -6  mol L -1 . Parkinson's disease protein 7 (PARK7/DJ-1) was successfully detected by electrochemical impedance spectroscopy after electrode functionalization with specific anti-PARK7/DJ-1 antibodies. In this case, analytical curves presented a linear behavior from 40 ng mL -1 to 150 ng mL -1 of PARK7/DJ-1 with a limit of detection of 7.5 ng mL -1 . Besides, the electrodes did not suffer any change in the electrochemical response after manual tests of mechanical tension. The proposed sensor and immunosensor were applied for the determination of Parkinson's disease biomarkers concentrations found in the human body, being adequate as an alternative method to diagnose this disease.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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