Examination of Carbohydrate Products in Feces Reveals Potential Biomarkers Distinguishing Exclusive and Nonexclusive Breastfeeding Practices in Infants.

Autor:	Ranque CL; Department of Chemistry, University of California, Davis, CA, USA., Stroble C; Department of Chemistry, University of California, Davis, CA, USA., Amicucci MJ; Department of Chemistry, University of California, Davis, CA, USA., Tu D; Department of Chemistry, University of California, Davis, CA, USA., Diana A; Department of Human Nutrition, University of Otago, Dunedin, New Zealand.; Nutrition Working Group, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia., Rahmannia S; Nutrition Working Group, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.; Faculty of Medicine, Universitas Pasundan, Bandung, Indonesia., Suryanto AH; Nutrition Working Group, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia., Gibson RS; Department of Human Nutrition, University of Otago, Dunedin, New Zealand., Sheng Y; Department of Chemistry, University of California, Davis, CA, USA., Tena J; Department of Chemistry, University of California, Davis, CA, USA., Houghton LA; Department of Human Nutrition, University of Otago, Dunedin, New Zealand., Lebrilla CB; Department of Chemistry, University of California, Davis, CA, USA.
Jazyk:	angličtina
Zdroj:	The Journal of nutrition [J Nutr] 2020 May 01; Vol. 150 (5), pp. 1051-1057.
DOI:	10.1093/jn/nxaa028
Abstrakt:	Background: The stable isotope deuterium dose-to-mother (DTM) technique to estimate nonbreast milk water intake demonstrates that maternal self-report methods of infant feeding overestimate the true prevalence of exclusively breastfeeding practices. Objective: We aimed to determine potential monosaccharide and oligosaccharide markers that distinguish between exclusively breastfed (EBF) versus nonexclusively breastfed (non-EBF) infants utilizing LC-MS-based methods. Methods: Data for the analysis were collected as part of a larger, longitudinal study of 192 breastfed Indonesian infants aged 2 mo and followed up at 5 mo. Feces samples were collected from infants aged 2 mo (n = 188) and 5 mo (n = 184). EBF and non-EBF strata at each time point were determined via the DTM technique. Feces samples were analyzed to determine monosaccharide content using ultra-high-performance LC-triple quadrupole MS (UHPLC-QqQ MS). Relative abundances of fecal oligosaccharides were determined using nano-LC-Chip-quadrupole time-of-flight MS (nano-LC-Chip-Q-ToF MS). Results: At age 2 mo, monosaccharide analysis showed the abundance of fructose and mannose were significantly higher (+377% and +388%, respectively) in non-EBF compared with EBF infants (P <0.0001). Fructose and mannose also showed good discrimination with areas under the curve (AUC) of 0.86 and 0.82, respectively. Oligosaccharide analysis showed that a 6-hexose (Hex6) isomer had good discrimination (AUC = 0.80) between EBF and non-EBF groups at 5 mo. Conclusion: Carbohydrate products, particularly fecal mono- and oligosaccharides, differed between EBF and non-EBF infants aged under 6 mo and can be used as potential biomarkers to distinguish EBF versus non-EBF feeding practices. (Copyright © The Author(s) 2020.)
Databáze:	MEDLINE
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