Comparative lipidomic analysis of inflammatory mediators in the aqueous humor and tear fluid of humans and rabbits.

Autor: Chistyakov DV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992. chistyakof@gmail.com., Azbukina NV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992.; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia, 119234., Astakhova AA; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992., Goriainov SV; Peoples' Friendship University of Russia, Moscow, Russia, 117198., Chistyakov VV; Peoples' Friendship University of Russia, Moscow, Russia, 117198., Tiulina VV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992., Baksheeva VE; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992., Kotelin VI; Helmholtz National Medical Research Center of Eye Diseases, Moscow, Russia, 105062., Fedoseeva EV; Helmholtz National Medical Research Center of Eye Diseases, Moscow, Russia, 105062., Zamyatnin AA Jr; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992.; Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, Russia, 119991., Philippov PP; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992., Kiseleva OA; Helmholtz National Medical Research Center of Eye Diseases, Moscow, Russia, 105062., Bessmertny AM; Helmholtz National Medical Research Center of Eye Diseases, Moscow, Russia, 105062., Senin II; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992., Iomdina EN; Helmholtz National Medical Research Center of Eye Diseases, Moscow, Russia, 105062., Sergeeva MG; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992., Zernii EY; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskye Gory, Moscow, Russia, 119992. zerni@belozersky.msu.ru.; Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, Russia, 119991. zerni@belozersky.msu.ru.
Jazyk: angličtina
Zdroj: Metabolomics : Official journal of the Metabolomic Society [Metabolomics] 2020 Feb 12; Vol. 16 (2), pp. 27. Date of Electronic Publication: 2020 Feb 12.
DOI: 10.1007/s11306-020-1650-y
Abstrakt: Introduction: Ocular inflammation is a key pathogenic factor in most blindness-causing visual disorders. It can manifest in the aqueous humor (AH) and tear fluid (TF) as alterations in polyunsaturated fatty acids (PUFAs) and their metabolites, oxylipins, lipid mediators, which are biosynthesized via enzymatic pathways involving lipoxygenase, cyclooxygenase or cytochrome P450 monooxygenase and specifically regulate inflammation and resolution pathways.
Objectives: This study aimed to establish the baseline patterns of PUFAs and oxylipins in AH and TF by their comprehensive lipidomic identification and profiling in humans in the absence of ocular inflammation and comparatively analyze these compounds in the eye liquids of rabbits, the species often employed in investigative ophthalmology.
Methods: Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used for qualitative and quantitative characterization of lipid compounds in the analyzed samples.
Results: A total of 28 lipid compounds were identified, including phospholipid derivatives and PUFAs, as well as 22 oxylipins. Whereas the PUFAs included arachidonic, docosahexaenoic and eicosapentaenoic acids, the oxylipins were derived mainly from arachidonic, linoleic and α-linolenic acids. Remarkably, although the concentration of oxylipins in AH was lower compared to TF, these liquids showed pronounced similarity in their lipid profiles, which additionally exhibited noticeable interspecies concordance.
Conclusion: The revealed correlations confirm the feasibility of rabbit models for investigating pathogenesis and trialing therapies of human eye disorders. The identified metabolite patterns suggest enzymatic mechanisms of oxylipin generation in AH and TF and might be used as a reference in ocular inflammation studies.
Databáze: MEDLINE
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