Antiviral activity of curcumin-nanoemulsion associated with photodynamic therapy in vulvar cell lines transducing different variants of HPV-16.

Autor: Bonfim CMD; Laboratory of Genomic Studies, Sao Paulo State University - UNESP, São Paulo, Brazil., Monteleoni LF; Laboratory of Genomic Studies, Sao Paulo State University - UNESP, São Paulo, Brazil., Calmon MF; Laboratory of Genomic Studies, Sao Paulo State University - UNESP, São Paulo, Brazil., Cândido NM; Laboratory of Genomic Studies, Sao Paulo State University - UNESP, São Paulo, Brazil., Provazzi PJS; Laboratory of Genomic Studies, Sao Paulo State University - UNESP, São Paulo, Brazil., Lino VS; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo - USP, São Paulo, Brazil., Rabachini T; Institute of Pharmacology, University of Bern, Bern, Switzerland., Sichero L; ICESP, Center for Translational Research in Oncology- Instituto do Cancer do Estado de Sao Paulo, São Paulo, Brazil., Villa LL; ICESP, Center for Translational Research in Oncology- Instituto do Cancer do Estado de Sao Paulo, São Paulo, Brazil.; Departament of Radiology and Oncology, School of Medicine, Universidade de Sao Paulo - USP, São Paulo, Brazil., Quintana SM; Department of Gynecology and Obstetrics of the Ribeirão Preto Medical School, USP, Ribeirão Preto, São Paulo, Brazil., Melli PPDS; Department of Gynecology and Obstetrics of the Ribeirão Preto Medical School, USP, Ribeirão Preto, São Paulo, Brazil., Primo FL; Department of Bioprocess and Biotechnology, Faculty of Pharmaceutical Sciences of Araraquara, Universidade Estadual Paulista Julio de Mesquita Filho - UNESP, Araraquara, São Paulo, Brazil., Amantino CF; Department of Chemistry, Center for Nanotechnology and Tissue Engineering, Faculty of Philosophy, Sciences and Languages ​​of Ribeirão Preto, University of São Paulo - USP, Ribeirão Preto, São Paulo, Brazil., Tedesco AC; Department of Chemistry, Center for Nanotechnology and Tissue Engineering, Faculty of Philosophy, Sciences and Languages ​​of Ribeirão Preto, University of São Paulo - USP, Ribeirão Preto, São Paulo, Brazil., Boccardo E; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo - USP, São Paulo, Brazil., Rahal P; Laboratory of Genomic Studies, Sao Paulo State University - UNESP, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Artificial cells, nanomedicine, and biotechnology [Artif Cells Nanomed Biotechnol] 2020 Dec; Vol. 48 (1), pp. 515-524.
DOI: 10.1080/21691401.2020.1725023
Abstrakt: Vulvar intraepithelial neoplasia (VIN) is associated with human papillomavirus (HPV) infection. Curcumin is a natural bioactive compound with antineoplastic properties. The use of nanoparticles containing curcumin could allow a better performance of this compound in therapies. So, VIN biopsies were collected and HPV DNA detection was performed by PCR, positive samples were genotyped by Restriction Fragment Length Polymorphism (RFLP) and HPV-16 variants were determined by sequencing. HPV-16 positive vulva carcinoma cells (A431) were transduced with E-P and E-350G HPV-16 E6 variants. The viability of the transduced cells treated with nanoemulsions was determined by MTT assay. Besides, apoptosis was evaluated by enzymatic activity of Caspase-3/7. The cell viability assay showed that both the empty nanoemulsion (NE-V) and the nanoemulsion of curcumin (NE-CUR) had little effect on cell viability as compared to control cells. Additionally, we observed that cells irradiated in the presence of NE-CUR presented 90% of cell death. The apoptosis assay further revealed a significant increase in the activity of caspases 3 and 7 in A431 cells expressing both HPV-16 E6 variants after treatment with NE-CUR. Finally, we submitted the HPV transduced A431 cells to organotypic cultures and observed that the combination of treatments affected tissue architecture with evident signals of tissue damage. We concluded that nanoemulsions attain good biocompatibility, since no cytotoxicity was observed and NE-CUR associated with photoactivation showed promising results, leading to death only in cells subjected to irradiation. This drug delivery system associated with photodynamic therapy may become promising in the treatment of vulva lesions.
Databáze: MEDLINE
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