Use of Alginate Extracted from Moroccan Brown Algae to Stimulate Natural Defense in Date Palm Roots.

Autor: Bouissil S; Université Cadi Ayyad, Laboratoire de Biotechnologie et Bioingénierie Moléculaire, Faculté des Sciences et Techniques Marrakech, Marrakech 40000, Morocco.; Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France., El Alaoui-Talibi Z; Université Cadi Ayyad, Laboratoire de Biotechnologie et Bioingénierie Moléculaire, Faculté des Sciences et Techniques Marrakech, Marrakech 40000, Morocco., Pierre G; Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France., Michaud P; Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France., El Modafar C; Université Cadi Ayyad, Laboratoire de Biotechnologie et Bioingénierie Moléculaire, Faculté des Sciences et Techniques Marrakech, Marrakech 40000, Morocco., Delattre C; Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France.; Institut Universitaire de France (IUF),1 rue Descartes 75005 Paris, France.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2020 Feb 07; Vol. 25 (3). Date of Electronic Publication: 2020 Feb 07.
DOI: 10.3390/molecules25030720
Abstrakt: Our study aimed to search for seaweed polysaccharides able to stimulate date palm defense mechanisms. Extraction, purification, characterization, and elicitor activity of sodium alginate (FSSA and BBSA) from Moroccan brown seaweeds Fucus spiralis and Bifurcaria bifurcata were investigated. FSSA and BBSA were characterized by proton nuclear magnetic resonance spectroscopy ( 1 H-NMR) and size exclusion chromatography (HPLC-SEC). The mannuronic acid/guluronic acid (M/G) ratio of FSSA was M/G= 0.92 indicating that FSSA contained 48% and 52% of mannuronic and guluronic acids respectively, and the M/G ratio of BBSA was 0.47 indicating that BBSA contained 32% and 68% of mannuronic and guluronic acids respectively. Elicitor activity of FSSA and BBSA was carried out by developing an innovative study model on the date palm. The elicitor capacities were evaluated by investigating phenolic metabolism including phenylalanine ammonia-lyase (PAL) activity and total polyphenol content in seedling roots of date palm maintained in alginates solution (FSSA and BBSA) at different concentrations. The results obtained show that the PAL activity and the phenolic compound content were significantly stimulated with 1 mg.mL -1 of FSSA and BBSA; after 1 day of treatment with FSSA, and after 12 hours of treatment with BBSA. These results show clearly those alginates extracted from Moroccan brown algae induced in date palm roots the stimulation of natural defense mechanisms.
Competing Interests: The authors declare no conflict of interest, financial or otherwise.
Databáze: MEDLINE
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