Modulation of Living Cell Behavior with Ultra-Low Fouling Polymer Brush Interfaces.

Autor: Víšová I; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic., Smolková B; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic., Uzhytchak M; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic., Vrabcová M; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic., Zhigunova Y; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic., Houska M; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic., Surman F; Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 00, Prague, Czech Republic., de Los Santos Pereira A; Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 00, Prague, Czech Republic., Lunov O; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic., Dejneka A; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic., Vaisocherová-Lísalová H; Institute of Physics, Czech Academy of Sciences, Na Slovance 2, 182 21, Prague, Czech Republic.
Jazyk: angličtina
Zdroj: Macromolecular bioscience [Macromol Biosci] 2020 Mar; Vol. 20 (3), pp. e1900351. Date of Electronic Publication: 2020 Feb 11.
DOI: 10.1002/mabi.201900351
Abstrakt: Ultra-low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra-low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied. The state-of-art ultra-low fouling surface-grafted polymer brushes of zwitterionic poly(carboxybetaine acrylamide), nonionic poly(N-(2-hydroxypropyl)methacrylamide), and random copolymers of carboxybetaine methacrylamide (CBMAA) and HPMAA [p(CBMAA-co-HPMAA)] with tunable molar contents of CBMAA and HPMAA are employed. Using a model Huh7 cell line, a systematic study of surface wettability, swelling, and charge effects on the cell growth, shape, and cytoskeleton distribution is performed. This study reveals that ultra-low fouling interfaces with a high content of zwitterionic moieties (>65 mol%) modulate cell behavior in a distinctly different way compared to coatings with a high content of nonionic HPMAA. These differences are attributed mostly to the surface hydration capabilities. The results demonstrate a high potential of carboxybetaine-rich ultra-low fouling surfaces with high hydration capabilities and minimum background signal interferences to create next-generation bioresponsive interfaces for advanced studies of living objects.
(© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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