Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO 3 ) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus.

Autor: Ersoy SC; The Lundquist Institute, Torrance, California, USA., Otmishi M; California State University Dominguez Hills, Carson, California, USA., Milan VT; The Lundquist Institute, Torrance, California, USA., Li L; The Lundquist Institute, Torrance, California, USA., Pak Y; The Lundquist Institute, Torrance, California, USA., Mediavilla J; Meridian Health, Nutley, New Jersey, USA., Chen L; Meridian Health, Nutley, New Jersey, USA., Kreiswirth B; Meridian Health, Nutley, New Jersey, USA., Chambers HF; UCSF School of Medicine, San Francisco, California, USA., Proctor RA; University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA., Xiong YQ; The Lundquist Institute, Torrance, California, USA.; Geffen School of Medicine at UCLA, Los Angeles, California, USA., Fowler VG; Duke University Medical Center, School of Medicine, Durham, North Carolina, USA., Bayer AS; The Lundquist Institute, Torrance, California, USA abayer@lundquist.org.; Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Jazyk: angličtina
Zdroj: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2020 Apr 21; Vol. 64 (5). Date of Electronic Publication: 2020 Apr 21 (Print Publication: 2020).
DOI: 10.1128/AAC.02445-19
Abstrakt: Addition of sodium bicarbonate (NaHCO 3 ) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO 3 responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO 3 responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO 3 responsiveness for OXA.
(Copyright © 2020 Ersoy et al.)
Databáze: MEDLINE
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