Disulfide Crosslinked Hydrogels Made From the Hydra Stinging Cell Protein, Minicollagen-1.

Autor:	Farajollahi S; Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States.; UES Inc., Dayton, OH, United States., Dennis PB; Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States., Crosby MG; Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States., Slocik JM; Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States.; UES Inc., Dayton, OH, United States., Pelton AT; Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States.; UES Inc., Dayton, OH, United States., Hampton CM; Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States.; UES Inc., Dayton, OH, United States., Drummy LF; Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States., Yang SJ; Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, United States., Silberstein MN; Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, United States., Gupta MK; Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States., Naik RR; 711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in chemistry [Front Chem] 2020 Jan 23; Vol. 7, pp. 950. Date of Electronic Publication: 2020 Jan 23 (Print Publication: 2019).
DOI:	10.3389/fchem.2019.00950
Abstrakt:	Minicollagens from cnidarian nematocysts are attractive potential building blocks for the creation of strong, lightweight and tough polymeric materials with the potential for dynamic and reconfigurable crosslinking to modulate functionality. In this study, the Hydra magnipapillata minicollagen-1 isoform was recombinantly expressed in bacteria, and a high throughput purification protocol was developed to generate milligram levels of pure protein without column chromatography. The resulting minicollagen-1 preparation demonstrated spectral properties similar to those observed with collagen and polyproline sequences as well as the ability to self-assemble into oriented fibers and bundles. Photo-crosslinking with Ru(II) ( bpy ) 3 2 + was used to create robust hydrogels that were analyzed by mechanical testing. Interestingly, the minicollagen-1 hydrogels could be dissolved with reducing agents, indicating that ruthenium-mediated photo-crosslinking was able to induce disulfide metathesis to create the hydrogels. Together, this work is an important first step in creating minicollagen-based materials whose properties can be manipulated through static and reconfigurable post-translational modifications. (Copyright © 2020 Farajollahi, Dennis, Crosby, Slocik, Pelton, Hampton, Drummy, Yang, Silberstein, Gupta and Naik.)
Databáze:	MEDLINE
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