Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection.

Autor:	Lukianova AA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.; Department of Biology, Lomonosov Moscow State University, Moscow, Russia., Shneider MM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Evseev PV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Shpirt AM; Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Bugaeva EN; Research Center 'PhytoEngineering' Ltd., Rogachevo, Moscow, Russia., Kabanova AP; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.; Research Center 'PhytoEngineering' Ltd., Rogachevo, Moscow, Russia., Obraztsova EA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Miroshnikov KK; Winogradsky Institute of Microbiology, Federal Research Center 'Fundamentals of Biotechnology', Russian Academy of Sciences, Moscow, Russia., Senchenkova SN; Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Shashkov AS; Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Toschakov SV; Winogradsky Institute of Microbiology, Federal Research Center 'Fundamentals of Biotechnology', Russian Academy of Sciences, Moscow, Russia., Knirel YA; Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Ignatov AN; Research Center 'PhytoEngineering' Ltd., Rogachevo, Moscow, Russia., Miroshnikov KA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2020 Jan 23; Vol. 10, pp. 3147. Date of Electronic Publication: 2020 Jan 23 (Print Publication: 2019).
DOI:	10.3389/fmicb.2019.03147
Abstrakt:	Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of their infection mechanism for more rational composition of therapeutic cocktails. We present the study of two bacteriophages, infective for the Pectobacterium brasiliense strain F152. Podoviridae PP99 is a representative of the genus Zindervirus , and Myoviridae PP101 belongs to the still unclassified genomic group. The structure of O-polysaccharide of F152 was established by sugar analysis and 1D and 2D NMR spectroscopy: → 4)-α-D-Man p 6Ac-(1→ 2)-α-D-Man p -(1→ 3)-β-D-Gal p -(1→ 3 ↑ 1 α -l- 6 dTal p Ac 0 - 2 The recombinant tail spike protein of phage PP99, gp55, was shown to deacetylate the side chain talose residue of bacterial O-polysaccharide, thus providing the selective attachment of the phage to the cell surface. Both phages demonstrate lytic behavior, thus being prospective for therapeutic purposes. (Copyright © 2020 Lukianova, Shneider, Evseev, Shpirt, Bugaeva, Kabanova, Obraztsova, Miroshnikov, Senchenkova, Shashkov, Toschakov, Knirel, Ignatov and Miroshnikov.)
Databáze:	MEDLINE
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