Biological and physicochemical implications of the aging process on titanium and zirconia implant material surfaces.

Autor: da Rocha JFSS; Masters graduate, Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, Brazil., de Avila ED; Postdoctoral Research Fellow, Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, Brazil. Electronic address: erica.fobusp@yahoo.com.br., Rigolin MSM; Postdoctoral Research Fellow, Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, Brazil., Barbugli PA; Technical Assistant, Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, Brazil., Marin DOM; Assistant Professor, Department of Dentistry, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil., Mollo Junior FA; Associate Professor, Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, Brazil., Jorge JH; Associate Professor, Department of Dental Materials and Prosthodontics, School of Dentistry at Araraquara, São Paulo State University (UNESP), Araraquara, Brazil.
Jazyk: angličtina
Zdroj: The Journal of prosthetic dentistry [J Prosthet Dent] 2021 Jan; Vol. 125 (1), pp. 165-174. Date of Electronic Publication: 2020 Feb 07.
DOI: 10.1016/j.prosdent.2019.11.024
Abstrakt: Statement of Problem: Changes in physicochemical properties because of implant material aging and natural deterioration in the oral environment can facilitate microbial colonization and disturb the soft-tissue seal between the implant surfaces.
Purpose: The purpose of this in vitro study was to investigate the effect of aging time on the physicochemical profile of titanium (Ti) and zirconia (ZrO 2 ) implant materials. Further microbiology and cell analyses were used to provide insights into the physicochemical implications of biological behavior.
Material and Methods: Disk-shaped specimens of Ti and ZrO 2 were submitted to roughness, morphology, and surface free energy (SFE) analyses before nonaging (NA) and after the aging process (A). To simulate natural aging, disks were subjected to low-temperature degradation (LTD) by using an autoclave at 134 ºC and 0.2 MPa pressure for 20 hours. The biological activities of the Ti and ZrO 2 surfaces were determined by analyzing Candida albicans (C. albicans) biofilms and human gingival fibroblast (HGF) cell proliferation. For the microbiology assays, a variance analysis method (ANOVA) was used with the Tukey post hoc test. For the evaluation of cellular proliferation, the Kruskal-Wallis test followed by Dunn multiple comparisons were used.
Results: Ti nonaging (TNA) and ZrO 2 nonaging (ZNA) disks displayed hydrophilic and lipophilic properties, and this effect was sustained after the aging process. Low-temperature degradation resulted in a modest change in intermolecular interaction, with 1.06-fold for TA and 1.10-fold for ZA. No difference in biofilm formation was observed between NA and A disks of the same material. After 48 hours, the viability of the attached HGF cells was very similar to that in the NA and A groups, regardless of the tested material.
Conclusion: The changes in the physicochemical properties of Ti and ZrO 2 induced by the aging process do not interfere with C. albicans biofilm formation and HGF cell attachment, even after long-term exposure.
(Copyright © 2020 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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