Functional analysis of Ultrabithorax in the wing-dimorphic planthopper Nilaparvata lugens (Stål, 1854) (Hemiptera: Delphacidae).

Autor: Fu SJ; State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China; Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou 310058, China; Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China., Zhang JL; State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China; Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou 310058, China; Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China., Chen SJ; State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China; Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou 310058, China; Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China., Chen HH; State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China; Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou 310058, China; Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China., Liu YL; State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China; Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou 310058, China; Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China., Xu HJ; State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou 310058, China; Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, Hangzhou 310058, China; Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: haijunxu@zju.edu.cn.
Jazyk: angličtina
Zdroj: Gene [Gene] 2020 May 05; Vol. 737, pp. 144446. Date of Electronic Publication: 2020 Feb 05.
DOI: 10.1016/j.gene.2020.144446
Abstrakt: The homeotic complex (Hox) gene Ultrabithorax (Ubx) plays pivotal roles in modifying specific morphological differences among the second (T2), the third thoracic (T3), and the first abdomen (A1) segment in several insects. Whether Ubx regulates wing dimorphism and other morphological traits in the delphacid family (order Hemiptera) remains elusive. In this study, we cloned a full-length Ubx ortholog (NlUbx) from the wing-dimorphic planthopper Nilaparvata lugens, and identified two NlUbx isoforms. RNA-interference (RNAi)-mediated silencing of NlUbx in short-winged BPH nymphs significantly induced the development of wing-like appendages from T3 wingbuds, and this effect is likely mediated by the insulin/insulin-like signaling pathway. RNAi knockdown of NlUbx in long-winged BPH nymphs led to a transformation from hindwings to forewings. Additionally, silencing of NlUbx not only dramatically changed the T3 morphology, but also led to jumping defect of T3 legs. First-instar nymphs derived from parental RNAi had an additional leg-like appendages on A1. These results suggest that Ubx plays a role in determining some morphological traits in delphacid planthoppers, and thus help in understanding evolution of morphological characteristics in arthropods.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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