A novel CNS-homing peptide for targeting neuroinflammatory lesions in experimental autoimmune encephalomyelitis.

Autor: Acharya B; University of Maryland School of Medicine, Baltimore, MD, USA; Baltimore VA Medical Center, Baltimore, MD, USA., Meka RR; University of Maryland School of Medicine, Baltimore, MD, USA; Baltimore VA Medical Center, Baltimore, MD, USA., Venkatesha SH; University of Maryland School of Medicine, Baltimore, MD, USA; Baltimore VA Medical Center, Baltimore, MD, USA., Lees JR; Uniformed Services University of Health Sciences (USUHS), Bethesda, MD, USA., Teesalu T; Institute of Biomedicine and Translational Medicine, University of Tartu (UT), Estonia; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA., Moudgil KD; University of Maryland School of Medicine, Baltimore, MD, USA; Baltimore VA Medical Center, Baltimore, MD, USA. Electronic address: https://webmail.umaryland.edu/src/compose.php?send_to=kmoud001%40umaryland.edu.
Jazyk: angličtina
Zdroj: Molecular and cellular probes [Mol Cell Probes] 2020 Jun; Vol. 51, pp. 101530. Date of Electronic Publication: 2020 Feb 05.
DOI: 10.1016/j.mcp.2020.101530
Abstrakt: Using phage peptide library screening, we identified peptide-encoding phages that selectively home to the inflamed central nervous system (CNS) of mice with experimental autoimmune encephalomyelitis (EAE), a model of human multiple sclerosis (MS). A phage peptide display library encoding cyclic 9-amino-acid random peptides was first screened ex-vivo for binding to the CNS tissue of EAE mice, followed by in vivo screening in the diseased mice. Phage insert sequences that were present at a higher frequency in the CNS of EAE mice than in the normal (control) mice were identified by DNA sequencing. One of the phages selected in this manner, denoted as MS-1, was shown to selectively recognize CNS tissue in EAE mice. Individually cloned phages with this insert preferentially homed to EAE CNS after an intravenous injection. Similarly, systemically-administered fluorescence-labeled synthetic MS-1 peptide showed selective accumulation in the spinal cord of EAE mice. We suggest that peptide MS-1 might be useful for targeted drug delivery to CNS in EAE/MS.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Published by Elsevier Ltd.)
Databáze: MEDLINE
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