Skin Irritation Testing beyond Tissue Viability: Fucoxanthin Effects on Inflammation, Homeostasis, and Metabolism.

Autor: Spagolla Napoleão Tavares R; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café s/n, Monte Hosana Maria Debonsi Alegre, Ribeirão Preto, SP 14040-903, Brazil., Maria-Engler SS; Clinical and Toxicological Analyses Department, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 748, Cidade Universitária, São Paulo, SP 05508-000, Brazil., Colepicolo P; Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748, Cidade Universitária, São Paulo, SP 05508-000, Brazil., Debonsi HM; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café s/n, Monte Hosana Maria Debonsi Alegre, Ribeirão Preto, SP 14040-903, Brazil., Schäfer-Korting M; Institute of Pharmacy (Pharmacology & Toxicology), Freie Universität Berlin, Königin Luise Str 2+4, 14195 Berlin, Germany., Marx U; TissUse GmbH, Oudenarder Str. 16, 13347 Berlin, Germany., Gaspar LR; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. do Café s/n, Monte Hosana Maria Debonsi Alegre, Ribeirão Preto, SP 14040-903, Brazil., Zoschke C; Institute of Pharmacy (Pharmacology & Toxicology), Freie Universität Berlin, Königin Luise Str 2+4, 14195 Berlin, Germany.
Jazyk: angličtina
Zdroj: Pharmaceutics [Pharmaceutics] 2020 Feb 05; Vol. 12 (2). Date of Electronic Publication: 2020 Feb 05.
DOI: 10.3390/pharmaceutics12020136
Abstrakt: UV light catalyzes the ozone formation from air pollutants, like nitrogen oxides. Since ozone reacts with cutaneous sebum lipids to peroxides and, thus, promotes inflammation, tumorigenesis, and aging, even broad-spectrum sunscreens cannot properly protect skin. Meanwhile, xanthophylls, like fucoxanthin, proved their antioxidant and cytoprotective functions, but the safety of their topical application in human cell-based models remains unknown. Aiming for a more detailed insight into the cutaneous fucoxanthin toxicity, we assessed the tissue viability according to OECD test guideline no. 439 as well as changes in inflammation (IL-1α, IL-6, IL-8), homeostasis (EGFR, HSPB1) and metabolism (NAT1). First, we proved the suitability of our 24-well-based reconstructed human skin for irritation testing. Next, we dissolved 0.5% fucoxanthin either in alkyl benzoate or in ethanol and applied both solutions onto the tissue surface. None of the solutions decreased RHS viability below 50%. In contrast, fucoxanthin ameliorated the detrimental effects of ethanol and reduced the gene expression of pro-inflammatory interleukins 6 and 8, while increasing NAT1 gene expression. In conclusion, we developed an organ-on-a-chip compatible RHS, being suitable for skin irritation testing beyond tissue viability assessment. Fucoxanthin proved to be non-irritant in RHS and already showed first skin protective effects following topical application.
Competing Interests: The authors declare no conflict of interest. Uwe Marx is employee of the TissUse GmbH. Neither the company nor the funding source had a role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
Databáze: MEDLINE
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