A Comparative Transcriptome Analysis of Volvariella volvacea Identified the Candidate Genes Involved in Fast Growth at the Mycelial Growth Stage.

Autor:	Liu M; Vegetables Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China., Yu T; Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China., Singh PK; School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China., Liu Q; Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China., Liu H; School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China., Zhu Q; Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China., Xiao Z; Vegetables Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China., Xu J; Vegetables Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China., Peng Y; Vegetables Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China., Fu S; School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China., Chen S; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA., He H; Vegetables Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China.
Jazyk:	angličtina
Zdroj:	Genes [Genes (Basel)] 2020 Feb 04; Vol. 11 (2). Date of Electronic Publication: 2020 Feb 04.
DOI:	10.3390/genes11020161
Abstrakt:	The edible straw mushroom, Volvariella volvacea , is one of the most important cultivated mushrooms in tropical and sub-tropical regions. Strain improvement for V. volvacea is difficult because of the unknown mechanisms involved in its growth regulation and substrate utilization. A comparative physiological and transcriptomic study was conducted between two commercially available straw mushroom strains (v9 and v26) to explore their fast-growth regulation mechanism(s). The physiological study showed that V. volvacea v9 had a shorter growth cycle and higher biological efficiency (4% higher) than that in v26. At least 14,556 unigenes were obtained from the four cDNA libraries (two replicates per strain). Among them, the expression of 1597 unigenes was up-regulated while 1352 were down-regulated. Four heat-shock proteins were highly expressed in v9, showing that v9 has the better ability to handle stresses and/or environmental changes. Moreover, up to 14 putative transporter genes were expressed at a higher level in v9 than those in v26, implying that v9 has a better ability to transport nutrients or export xenobiotics efficiently. Our report allows to identify the candidate genes involved in the fast growth requirement of V. volvacea, which represents a valuable resource for strain improvement in this commercially important edible mushroom. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu