Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regulators of Adult Neurogenesis.

Autor:	Kjell J; Division of Physiological Genomics, Biomedical Center, Ludwig-Maximilians-Universitaet, Muenchen, Germany; Institute for Stem Cell Research, Helmholtz Zentrum Muenchen, Germany., Fischer-Sternjak J; Division of Physiological Genomics, Biomedical Center, Ludwig-Maximilians-Universitaet, Muenchen, Germany; Institute for Stem Cell Research, Helmholtz Zentrum Muenchen, Germany., Thompson AJ; Department of Physiology, Development and Neuroscience, Cambridge University, Cambridge, UK., Friess C; Division of Physiological Genomics, Biomedical Center, Ludwig-Maximilians-Universitaet, Muenchen, Germany., Sticco MJ; Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, USA., Salinas F; Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany., Cox J; Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany., Martinelli DC; Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, USA., Ninkovic J; Institute for Stem Cell Research, Helmholtz Zentrum Muenchen, Germany; Division of Cell Biology and Anatomy, Biomedical Center, Ludwig-Maximilians-Universitaet, Muenchen, Germany; SYNERGY, Excellence Cluster Systems Neurology, Ludwig-Maximilians-Universitaet, Muenchen, Germany., Franze K; Department of Physiology, Development and Neuroscience, Cambridge University, Cambridge, UK., Schiller HB; Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany; Institute of Lung Biology and Disease, Member of the German Center for Lung Research, Helmholtz Zentrum Muenchen, Germany., Götz M; Division of Physiological Genomics, Biomedical Center, Ludwig-Maximilians-Universitaet, Muenchen, Germany; Institute for Stem Cell Research, Helmholtz Zentrum Muenchen, Germany; SYNERGY, Excellence Cluster Systems Neurology, Ludwig-Maximilians-Universitaet, Muenchen, Germany. Electronic address: magdalena.goetz@helmholtz-muenchen.de.
Jazyk:	angličtina
Zdroj:	Cell stem cell [Cell Stem Cell] 2020 Feb 06; Vol. 26 (2), pp. 277-293.e8.
DOI:	10.1016/j.stem.2020.01.002
Abstrakt:	The mammalian brain contains few niches for neural stem cells (NSCs) capable of generating new neurons, whereas other regions are primarily gliogenic. Here we leverage the spatial separation of the sub-ependymal zone NSC niche and the olfactory bulb, the region to which newly generated neurons from the sub-ependymal zone migrate and integrate, and present a comprehensive proteomic characterization of these regions in comparison to the cerebral cortex, which is not conducive to neurogenesis and integration of new neurons. We find differing compositions of regulatory extracellular matrix (ECM) components in the neurogenic niche. We further show that quiescent NSCs are the main source of their local ECM, including the multi-functional enzyme transglutaminase 2, which we show is crucial for neurogenesis. Atomic force microscopy corroborated indications from the proteomic analyses that neurogenic niches are significantly stiffer than non-neurogenic parenchyma. Together these findings provide a powerful resource for unraveling unique compositions of neurogenic niches. Competing Interests: Declaration of Interests The authors declare no competing interests. (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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