Effects of moderate and high intensity isocaloric aerobic training upon microvascular reactivity and myocardial oxidative stress in rats.

Autor: Paes L; University of Rio de Janeiro State, Rio de Janeiro, Laboratory for Clinical and Experimental Research on Vascular Biology, Rio de Janeiro, Brazil., Lima D; University of Rio de Janeiro State, Rio de Janeiro, Department of Pharmacology and Psychobiology, Rio de Janeiro, Brazil., Matsuura C; University of Rio de Janeiro State, Rio de Janeiro, Department of Pharmacology and Psychobiology, Rio de Janeiro, Brazil., de Souza MDG; University of Rio de Janeiro State, Rio de Janeiro, Laboratory for Clinical and Experimental Research on Vascular Biology, Rio de Janeiro, Brazil., Cyrino F; University of Rio de Janeiro State, Rio de Janeiro, Laboratory for Clinical and Experimental Research on Vascular Biology, Rio de Janeiro, Brazil., Barbosa C; University of Rio de Janeiro State, Rio de Janeiro, Laboratory for Clinical and Experimental Research on Vascular Biology, Rio de Janeiro, Brazil., Ferrão F; University of Rio de Janeiro State, Rio de Janeiro, Laboratory for Clinical and Experimental Research on Vascular Biology, Rio de Janeiro, Brazil., Bottino D; University of Rio de Janeiro State, Rio de Janeiro, Laboratory for Clinical and Experimental Research on Vascular Biology, Rio de Janeiro, Brazil., Bouskela E; University of Rio de Janeiro State, Rio de Janeiro, Laboratory for Clinical and Experimental Research on Vascular Biology, Rio de Janeiro, Brazil., Farinatti P; University of Rio de Janeiro State, Rio de Janeiro, Laboratory of Physical Activity and Health Promotion, Rio de Janeiro, Brazil.; Salgado de Oliveira University, Niteroi, Graduate Program in Sciences of Physical Activity, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Feb 07; Vol. 15 (2), pp. e0218228. Date of Electronic Publication: 2020 Feb 07 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0218228
Abstrakt: Systemic and central cardiovascular adaptations may vary in response to chronic exercise performed with different intensities and volumes. This study compared the effects of aerobic training with different intensities but equivalent volume upon microvascular reactivity in cremaster muscle and myocardial biomarkers of oxidative stress in Wistar rats. After peak oxygen uptake (VO2peak) assessment, rats (n = 24) were assigned into three groups: moderate-intensity exercise training (MI); high-intensity exercise training (HI); sedentary control (SC). Treadmill training occurred during 4 weeks, with exercise bouts matched by the energy expenditure (3.0-3.5 Kcal). Microvascular reactivity was assessed in vivo by intravital microscopy in cremaster muscle arterioles, while biomarkers of oxidative stress and eNOS expression were quantified at left ventricle and at aorta, respectively. Similar increasing vs. sedentary control group (SC) occurred in moderate intensity training group (MI) and high-intensity training group (HI) for endothelium-dependent vasodilation (10-4M: MI: 168.7%, HI: 164.6% vs. SC: 146.6%, P = 0.0004). Superoxide dismutase (SOD) (HI: 0.13 U/mg vs. MI: 0.09 U/mg and SC: 0.06 U/mg; P = 0.02), glutathione peroxidase (GPX) (HI: 0.00038 U/mg vs. MI: 0.00034 U/mg and SC: 0.00024 U/mg; P = 0.04), and carbonyl protein content (HI: 0.04 U/mg vs. MI: 0.03 U/mg and SC: 0.01 U/mg; P = 0.003) increased only in HI. No difference across groups was detected for catalase (CAT) (P = 0.12), Thiobarbituric acid reactive substances (TBARS) (P = 0.38) or eNOS expression in aorta (P = 0.44). In conclusion, higher exercise intensity induced greater improvements in myocardium antioxidant defenses, while gains in microvascular reactivity appeared to rely more on exercise volume than intensity.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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