Analysis of complete chloroplast genome sequence of Korean landrace Cymbidium goeringii .

Autor: Wang H; 1Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, 50463 Republic of Korea.; 2State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China., Park SY; 1Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, 50463 Republic of Korea., Song SH; 1Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, 50463 Republic of Korea., San ML; 1Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, 50463 Republic of Korea., Kim YC; 1Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, 50463 Republic of Korea., Ham TH; 3Department of Applied Bioscience, Konkuk University, Seoul, 05029 Republic of Korea.; 4Department of Agricultural Science, Korea National Open University, Seoul, 03087 Republic of Korea., Kim DY; Saemangeum BioCenter Co, 1044 Heomi-ro, Daeya-myeon, Gunsan, 54061 Republic of Korea., Kim TS; 4Department of Agricultural Science, Korea National Open University, Seoul, 03087 Republic of Korea., Lee J; 3Department of Applied Bioscience, Konkuk University, Seoul, 05029 Republic of Korea., Kwon SW; 1Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang, 50463 Republic of Korea.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2020 Jan; Vol. 10 (1), pp. 29. Date of Electronic Publication: 2020 Jan 06.
DOI: 10.1007/s13205-019-2020-1
Abstrakt: The complete chloroplast genome sequence of Korean Cymbidium goeringii acc . smg222 was analyzed. Based on a comparison with Chinese C. goeringii , losses of nine ndh subunits ( ndh A, ndh B, ndh C, ndh D, ndh E, ndh F, ndh H, ndh J, and ndh K), three protein-coding genes ( ycf 1-like, ycf 15, and ycf 68), six transfer RNAs, and one conserved open reading frame ( orf 42). In addition, 219 InDels (insertion or deletion) and 171 simple sequence repeats were observed. Twenty-Five of which InDel markers have been evaluated, that useful for distinguishing Korean and Chinese Cymbidium associations based on the polymorphisms of chloroplast genomes between Korean Cymbidium goeringii acc . smg222 and Chinese C. goeringii and evaluation of genetic diversity. Finally, the phylogenetic relationships of the 39 Korean and 22 Chinese species was constructed based on the five InDel markers of them and obtained high support, indicating that our data may be useful in resolving relationships in this genus. The information about chloroplast DNA structure and gene variants of C. goeringii acc. smg222 chloroplast genome will provide sufficient phylogenetic information for resolving evolutionary relationships. The molecular markers developed in here will contribute to further research of Cymbidium species and conservation of endemic Cymbidium species.
Competing Interests: Conflict of interestAll the authors declare that they have on conflict of interest in the publication.
(© King Abdulaziz City for Science and Technology 2020.)
Databáze: MEDLINE
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