Automated quantification of Epstein-Barr virus in whole blood for post-transplant lymphoproliferative disorders monitoring.

Autor: Salmona M; Université de Paris Diderot, INSERM U976, Paris, France. maud.salmona@aphp.fr.; Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France. maud.salmona@aphp.fr., Stefic K; Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France.; Université de Tours, INSERM U1259, Tours, France., Mahjoub N; Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France., de Fontbrune FS; Hematology and Transplantation Unit, Hôpital Saint-Louis, APHP, Paris, France., Maylin S; Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France., Simon F; Université de Paris Diderot, INSERM U976, Paris, France.; Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France., Scieux C; Université de Paris Diderot, INSERM U976, Paris, France.; Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France., Socié G; Université de Paris Diderot, INSERM U976, Paris, France.; Hematology and Transplantation Unit, Hôpital Saint-Louis, APHP, Paris, France., Mazeron MC; Université de Paris Diderot, INSERM U976, Paris, France.; Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France., LeGoff J; Université de Paris Diderot, INSERM U976, Paris, France.; Laboratoire de Microbiologie, Hôpital Saint-Louis, APHP, Paris, France.
Jazyk: angličtina
Zdroj: Virology journal [Virol J] 2020 Feb 03; Vol. 17 (1), pp. 20. Date of Electronic Publication: 2020 Feb 03.
DOI: 10.1186/s12985-020-1285-7
Abstrakt: Background: Standardized and sensitive assays for Epstein Barr Virus (EBV) are needed to define universal cutoff for treatment initiation in allogeneic hematopoietic stem cells transplant recipients. In a context of accreditation and the availability of EBV international standard, we evaluated the Abbott RealTime EBV (RT) assay for EBV quantification in whole blood.
Methods: The RT assay was compared on 282 prospective clinical samples with the Artus EBV PCR Kit V1 assay (V1) and we analyzed the kinetics of EBV load in 11 patients receiving rituximab treatment.
Results: The estimated limit of detection was 88 IU/mL. The assay was linear (r 2  = 0.9974) in the range of all samples tested (100 to 1,000,000 IU/mL). Intra-assay coefficients of variation (CV) ranged between 0.35 and 1.35%, and inter-assay CV between 3.40 and 4.5%. On samples above the limit of quantification, the two assays were strongly correlated. EBV RT values were on average 0.30 log 10 IU/mL lower than those measured with the V1 assay. In patients treated with rituximab, the RT assay remained positive in 5 patients at the time it dropped below undetectable levels with the V1 assay.
Conclusions: In conclusion, the RT assay is a reliable assay for EBV load in whole blood. Its sensitivity will enable to estimate the kinetics of EBV load and the impact of treatments to control EBV reactivations.
Databáze: MEDLINE
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