Prevalence of Bartonella spp. Infection in Patients with Sickle Cell Disease.

Autor:	Soares TCB; São Leopoldo Mandic Institute and Research Center, Campinas, Brazil., Isaias GAB; Applied Research in Dermatology and Bartonella Infection Laboratory, University of Campinas (UNICAMP) Medical School, Campinas, Sao Paulo, Brazil., Almeida AR; Applied Research in Dermatology and Bartonella Infection Laboratory, University of Campinas (UNICAMP) Medical School, Campinas, Sao Paulo, Brazil., Drummond MR; Applied Research in Dermatology and Bartonella Infection Laboratory, University of Campinas (UNICAMP) Medical School, Campinas, Sao Paulo, Brazil., da Silva MN; Applied Research in Dermatology and Bartonella Infection Laboratory, University of Campinas (UNICAMP) Medical School, Campinas, Sao Paulo, Brazil., Lania BG; Applied Research in Dermatology and Bartonella Infection Laboratory, University of Campinas (UNICAMP) Medical School, Campinas, Sao Paulo, Brazil., Vieira-Damiani G; Federal Institute of Education, Science and Technology of Sao Paulo, Capivari, Brazil., Saad STO; Hematology and Transfusion Medicine Center, University of Campinas (UNICAMP), Campinas, Brazil., Ericson ME; Department of Dermatology, University of Minnesota, Minneapolis, Minnesota, USA., Gupta K; Division of Hematology, Oncology and Transplantation, University of Minnesota, Minneapolis, Minnesota, USA., Velho PENF; Division of Dermatology, Department of Medicine, University of Campinas (UNICAMP) Medical School, Campinas, Brazil.
Jazyk:	angličtina
Zdroj:	Vector borne and zoonotic diseases (Larchmont, N.Y.) [Vector Borne Zoonotic Dis] 2020 Jul; Vol. 20 (7), pp. 509-512. Date of Electronic Publication: 2020 Feb 03.
DOI:	10.1089/vbz.2019.2545
Abstrakt:	Background: The inherent characteristics of the sickle cell disease (SCD), the most common genetic hematological disorder, increase the propensity of infections. Bartonella spp. are emerging and neglected bacteria. A large spectrum of clinical manifestations has been linked to bartonella bloodstream infection in the last two decades that can cause fatal outcomes, especially in immunodeficient patients. The goal of this study was to evaluate the prevalence of bartonella infection in SCD patients. Materials and Methods: We evaluated Bartonella spp. prevalence in 107 SCD patients. Blood samples and enrichment blood cultures were analyzed by molecular detection of Bartonella spp. DNA. Bartonella DNA was amplified using conventional genus-specific Bartonella PCR which amplifies the Intergenic Transcribed Spacer region and Bartonella henselae- specific nested PCR which amplifies the FtsZ gene. Positive patient DNAs were tested with ssrA conventional PCR. All amplicons were sequenced. Findings: Ten of 107 patients tested positive for B. henselae infection in at least one molecular test. All obtained amplicons were sequenced and similar to B. henselae sequences deposited in GenBank (accession number BX897699). Based on statistical results, bloodstream infection with B. henselae was not associated with animal contact or blood transfusions. Conclusion: We detected B. henselae DNA in 10 (9.3%) SCD studied patients. These patients were notified and treatment was offered to them.
Databáze:	MEDLINE
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