Phytochemical Screening and Antiprotozoal Effects of the Methanolic Berberis vulgaris and Acetonic Rhus coriaria Extracts.

Autor: Batiha GE; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan.; Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt., Magdy Beshbishy A; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan., Adeyemi OS; Department of Biochemistry, Medicinal Biochemistry, Nanomedicine and Toxicology Laboratory, Landmark University, Omu-Aran 251101, Kwara State, Nigeria., Nadwa EH; Department of Pharmacology and Therapeutics, College of Medicine, Jouf University 72345, Saudi Arabia.; Department of Medical Pharmacology, Faculty of Medicine, Cairo University, 12613, Egypt., Rashwan EKM; Department of Physiology, College of Medicine, Al-Azhar University, Assuit 71524, Egypt.; Department of Physiology, College of Medicine, Jouf University, Sakaka 42421, Saudi Arabia., Alkazmi LM; Biology department, Faculty of Applied Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia., Elkelish AA; Department of Botany, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt., Igarashi I; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, 080-8555, Obihiro, Hokkaido, Japan.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2020 Jan 27; Vol. 25 (3). Date of Electronic Publication: 2020 Jan 27.
DOI: 10.3390/molecules25030550
Abstrakt: Berberis vulgaris ( B. vulgaris ) and Rhus coriaria (R. coriaria) have been documented to have various pharmacologic activities. The current study assessed the in vitro as well as in vivo inhibitory efficacy of a methanolic extract of B. vulgaris (MEBV) and an acetone extract of R. coriaria (AERC) on six species of piroplasm parasites. The drug-exposure viability assay was tested on three different cell lines, namely mouse embryonic fibroblast (NIH/3T3), Madin-Darby bovine kidney (MDBK) and human foreskin fibroblast (HFF) cells. Qualitative phytochemical estimation revealed that both extracts containing alkaloid, tannin, saponins and terpenoids and significant amounts of flavonoids and polyphenols. The GC-MS analysis of MEBV and AERC revealed the existence of 27 and 20 phytochemical compounds, respectively. MEBV and AERC restricted the multiplication of Babesia (B.) bovis, B. bigemina, B. divergens, B. caballi, and Theileria (T.) equi at the half-maximal inhibitory concentration (IC 50 ) of 0.84 ± 0.2, 0.81 ± 0.3, 4.1 ± 0.9, 0.35 ± 0.1 and 0.68 ± 0.1 µg/mL and 85.7 ± 3.1, 60 ± 8.5, 90 ± 3.7, 85.7 ± 2.1 and 78 ± 2.1 µg/mL, respectively. In the cytotoxicity assay, MEBV and AERC inhibited MDBK, NIH/3T3 and HFF cells with half-maximal effective concentrations (EC 50 ) of 695.7 ± 24.9, 931 ± 44.9, ˃1500 µg/mL and 737.7 ± 17.4, ˃1500 and ˃1500 µg/mL, respectively. The experiments in mice showed that MEBV and AERC prohibited B. microti multiplication at 150 mg/kg by 66.7% and 70%, respectively. These results indicate the prospects of these extracts as drug candidates for piroplasmosis treatment following additional studies in some clinical cases.
Competing Interests: The authors declare that they have no competing interests.
Databáze: MEDLINE
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