Neuropeptide Y Enhances Progerin Clearance and Ameliorates the Senescent Phenotype of Human Hutchinson-Gilford Progeria Syndrome Cells.

Autor: Aveleira CA; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal., Ferreira-Marques M; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.; Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal., Cortes L; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal., Valero J; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal., Pereira D; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Institute for Interdisciplinary Research , University of Coimbra, Coimbra, Portugal., Pereira de Almeida L; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.; Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal., Cavadas C; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.; Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.
Jazyk: angličtina
Zdroj: The journals of gerontology. Series A, Biological sciences and medical sciences [J Gerontol A Biol Sci Med Sci] 2020 May 22; Vol. 75 (6), pp. 1073-1078.
DOI: 10.1093/gerona/glz280
Abstrakt: Hutchinson-Gilford progeria syndrome (HGPS, or classical progeria) is a rare genetic disorder, characterized by premature aging, and caused by a de novo point mutation (C608G) within the lamin A/C gene (LMNA), producing an abnormal lamin A protein, termed progerin. Accumulation of progerin causes nuclear abnormalities and cell cycle arrest ultimately leading to cellular senescence. Autophagy impairment is a hallmark of cellular aging, and the rescue of this proteostasis mechanism delays aging progression in HGPS cells. We have previously shown that the endogenous Neuropeptide Y (NPY) increases autophagy in hypothalamus, a brain area already identified as a central regulator of whole-body aging. We also showed that NPY mediates caloric restriction-induced autophagy. These results are in accordance with other studies suggesting that NPY may act as a caloric restriction mimetic and plays a role as a lifespan and aging regulator. The aim of the present study was, therefore, to investigate if NPY could delay HGPS premature aging phenotype. Herein, we report that NPY increases autophagic flux and progerin clearance in primary cultures of human dermal fibroblasts from HGPS patients. NPY also rescues nuclear morphology and decreases the number of dysmorphic nuclei, a hallmark of HGPS cells. In addition, NPY decreases other hallmarks of aging as DNA damage and cellular senescence. Altogether, these results show that NPY rescues several hallmarks of cellular aging in HGPS cells, suggesting that NPY can be considered a promising strategy to delay or block the premature aging of HGPS.
(© The Author(s) 2020. Published by Oxford University Press on behalf of The Gerontological Society of America.)
Databáze: MEDLINE