Genome comparison and transcriptome analysis of the invasive brown root rot pathogen, Phellinus noxius, from different geographic regions reveals potential enzymes associated with degradation of different wood substrates.

Autor: Ibarra Caballero JR; Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523, USA., Ata JP; Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523, USA; Department of Forest Biological Sciences, University of the Philippines Los Baños, Laguna 4031, Philippines., Leddy KA; Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523, USA., Glenn TC; Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA., Kieran TJ; Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA., Klopfenstein NB; USDA Forest Service, Rocky Mountain Research Station, Moscow, ID 83843, USA., Kim MS; USDA Forest Service, Pacific Northwest Research Station, Corvallis, OR 97331, USA. Electronic address: meesook.kim@usda.gov., Stewart JE; Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: Jane.Stewart@colostate.edu.
Jazyk: angličtina
Zdroj: Fungal biology [Fungal Biol] 2020 Feb; Vol. 124 (2), pp. 144-154. Date of Electronic Publication: 2020 Jan 08.
DOI: 10.1016/j.funbio.2019.12.007
Abstrakt: Phellinus noxius is a root-decay pathogen with a pan-tropical/subtropical distribution that attacks a wide range of tree hosts. For this study, genomic sequencing was conducted on P. noxius isolate P919-02W.7 from Federated States of Micronesia (Pohnpei), and its gene expression profile was analyzed using different host wood (Acer, Pinus, Prunus, and Salix) substrates. The assembled genome was 33.92 Mbp with 2954 contigs and 9389 predicted genes. Only small differences were observed in size and gene content in comparison with two other P. noxius genome assemblies (isolates OVT-YTM/97 from Hong Kong, China and FFPRI411160 from Japan, respectively). Genome analysis of P. noxius isolate P919-02W.7 revealed 488 genes encoding proteins related to carbohydrate and lignin metabolism, many of these enzymes are associated with degradation of plant cell wall components. Most of the transcripts expressed by P. noxius isolate P919-02W.7 were similar regardless of wood substrates. This study highlights the vast suite of decomposing enzymes produced by P. noxius, which suggests potential for degrading diverse wood substrates, even from temperate host trees. This information contributes to our understanding of pathogen ecology, mechanisms of wood decomposition, and pathogenic/saprophytic lifestyle.
(Copyright © 2020 British Mycological Society. All rights reserved.)
Databáze: MEDLINE
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