| Autor: |
Wang EA; Department of Tissue Growth and Repair, Genetics Institute, Cambridge, MA 02140., Rosen V, Cordes P, Hewick RM, Kriz MJ, Luxenberg DP, Sibley BS, Wozney JM |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1988 Dec; Vol. 85 (24), pp. 9484-8. |
| DOI: |
10.1073/pnas.85.24.9484 |
| Abstrakt: |
We purified a factor that induces bone formation greater than 300,000-fold from guanidinium chloride extracts of demineralized bone. Fifty nanograms of highly purified protein was active in an in vivo cartilage and bone-formation assay. The activity resided in a single gel band, corresponding to a molecular mass of approximately 30 kDa, which yielded proteins of 30, 18, and 16 kDa on reduction. The partial amino acid sequence obtained from these proteins confirmed our identification of specific factors that induce new bone formation in vivo. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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