Decrease of Arabidopsis PAO activity entails increased RBOH activity, ROS content and altered responses to Pseudomonas.

Autor: Jasso-Robles FI; Facultad de Ciencias, Universidad Autónoma de San Luis Potosí (UASLP), Av. Chapultepec 1570, Priv. del Pedregal, 78295, San Luis Potosí, Mexico. Electronic address: qfb.ignaciorobles@gmail.com., Gonzalez ME; Instituto Tecnológico de Chascomús (INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avda. Intendente Marino Km 8.2, 7130, Chascomús, Provincia de Buenos Aires, Argentina. Electronic address: mariaelisa@intech.gov.ar., Pieckenstain FL; Instituto Tecnológico de Chascomús (INTECH), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avda. Intendente Marino Km 8.2, 7130, Chascomús, Provincia de Buenos Aires, Argentina. Electronic address: pieckenstain@intech.gov.ar., Ramírez-García JM; Facultad de Ciencias, Universidad Autónoma de San Luis Potosí (UASLP), Av. Chapultepec 1570, Priv. del Pedregal, 78295, San Luis Potosí, Mexico. Electronic address: miguel_5rmz@hotmail.com., Guerrero-González ML; Facultad de Agronomía y Veterinaria, Universidad Autónoma de San Luis Potosí (UASLP), Carretera San Luis-Matehuala Km 14.5, Ejido Palma de la Cruz, 78321, Soledad de Graciano Sánchez, San Luis Potosí, Mexico. Electronic address: luz.guerrero@uaslp.mx., Jiménez-Bremont JF; División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica (IPICYT), Camino a la Presa de San José 2055, Lomas 4ª Sección, 78216, San Luis Potosí, Mexico. Electronic address: jbremont@ipicyt.edu.mx., Rodríguez-Kessler M; Facultad de Ciencias, Universidad Autónoma de San Luis Potosí (UASLP), Av. Chapultepec 1570, Priv. del Pedregal, 78295, San Luis Potosí, Mexico. Electronic address: mrodriguez@fc.uaslp.mx.
Jazyk: angličtina
Zdroj: Plant science : an international journal of experimental plant biology [Plant Sci] 2020 Mar; Vol. 292, pp. 110372. Date of Electronic Publication: 2019 Dec 17.
DOI: 10.1016/j.plantsci.2019.110372
Abstrakt: Polyamines (PAs) are small aliphatic amines with important regulatory activities in plants. Biotic stress results in changes in PA levels due to de novo synthesis and PA oxidation. In Arabidopsis thaliana five FAD-dependent polyamine oxidase enzymes (AtPAO1-5) participate in PA back-conversion and degradation. PAO activity generates H 2 O 2 , an important molecule involved in cell signaling, elongation, programmed cell death, and defense responses. In this work we analyzed the role of AtPAO genes in the Arabidopsis thaliana-Pseudomonas syringae pathosystem. AtPAO1 and AtPAO2 genes were transcriptionally up-regulated in infected plants. Atpao1-1 and Atpao2-1 single mutant lines displayed altered responses to Pseudomonas, and an increased susceptibility was found in the double mutant Atpao1-1 x Atpao2-1. These polyamine oxidases mutant lines showed disturbed contents of ROS (H 2 O 2 and O 2 - ) and altered activities of RBOH, CAT and SOD enzymes both in infected and control plants. In addition, changes in the expression levels of AtRBOHD, AtRBOHF, AtPRX33, and AtPRX34 genes were also noticed. Our data indicate an important role for polyamine oxidases in plant defense and ROS homeostasis.
Competing Interests: Declaration of Competing Interest The authors declare there is no conflict of interest.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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