| Autor: |
Sukhodub LB; Sumy State University, Sumy, Ukraine., Sukhodub LF; Sumy State University, Sumy, Ukraine., Kumeda MO; Sumy State University, Sumy, Ukraine., Prylutskyy YI; Taras Shevchenko National University of Kyiv, Kiev, Ukraine., Pogorielov MV; Sumy State University, Sumy, Ukraine., Evstigneev MP; Sevastopol State University, Sevastopol, Crimea. max_evstigneev@mail.ru., Kostjukov VV; Sevastopol State University, Sevastopol, Crimea., Strutynska NY; Taras Shevchenko National University of Kyiv, Kiev, Ukraine., Vovchenko LL; Taras Shevchenko National University of Kyiv, Kiev, Ukraine., Khrapatiy SV; Taras Shevchenko National University of Kyiv, Kiev, Ukraine., Ritter U; Technical University of Ilmenau, Ilmenau, Germany. |
| Abstrakt: |
Single-walled carbon nanotubes (SWCNTs) containing biomaterial with enhanced mechanical properties for the potential orthopedic application were synthesized and investigated. X-ray diffraction and X-ray fluorescence analysis were indications of the formation of calcium-deficient (Ca/P = 1.65) hydroxyapatite (HA) with a small carbonate content under influence of microwave irradiation. The investigated mechanical properties (maximal relative deformation, compressive strength and Young's modulus) of SWCNT loaded HA-alginate composites confirm their dependence on SWCNTs content. The compressive strength of HA-alginate-SWCNT and the HA-alginate control (202 and 159 MPa, respectively) lies within the values characteristic for the cortical bone. The addition of 0.5% SWCNT, in relation to the content of HA, increases the Young's modulus of the HA-alginate-SWCNT (645 MPa) compared to the SWCNT-free HA-alginate sample (563 MPa), and enhances the material shape stability in simulated physiological conditions. Structural modeling of HA-alginate-SWCNT system showed, that physical adsorption of SWCNT into HA-alginate occurs by forming triple complexes stabilized by solvophobic/van der Waals interactions and H-bonds. The high-performance liquid chromatography demonstrated the influence of SWCNTs on the sustained anaesthesinum drug (used as a model drug) release (456 h against 408 h for SWCNT-free sample). Cell culture assay confirmed biocompatibility and stimulation of osteoblast proliferation of 0.05% and 0.5% SWCNT-containing composites during a 3-day cultivation. All these facts may suggest the potential possibility of using the SWCNT-containing materials, based on HA and alginate, for bone tissue engineering. |
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