Engineered symbionts activate honey bee immunity and limit pathogens.

Autor: Leonard SP; Department of Integrative Biology, The University of Texas at Austin, Austin, TX 78712, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA., Powell JE; Department of Integrative Biology, The University of Texas at Austin, Austin, TX 78712, USA., Perutka J; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA., Geng P; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA., Heckmann LC; Department of Integrative Biology, The University of Texas at Austin, Austin, TX 78712, USA., Horak RD; Department of Integrative Biology, The University of Texas at Austin, Austin, TX 78712, USA., Davies BW; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA., Ellington AD; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA., Barrick JE; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA. nancy.moran@austin.utexas.edu jbarrick@cm.utexas.edu., Moran NA; Department of Integrative Biology, The University of Texas at Austin, Austin, TX 78712, USA. nancy.moran@austin.utexas.edu jbarrick@cm.utexas.edu.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2020 Jan 31; Vol. 367 (6477), pp. 573-576. Date of Electronic Publication: 2020 Jan 30.
DOI: 10.1126/science.aax9039
Abstrakt: Honey bees are essential pollinators threatened by colony losses linked to the spread of parasites and pathogens. Here, we report a new approach for manipulating bee gene expression and protecting bee health. We engineered a symbiotic bee gut bacterium, Snodgrassella alvi , to induce eukaryotic RNA interference (RNAi) immune responses. We show that engineered S. alvi can stably recolonize bees and produce double-stranded RNA to activate RNAi and repress host gene expression, thereby altering bee physiology, behavior, and growth. We used this approach to improve bee survival after a viral challenge, and we show that engineered S. alvi can kill parasitic Varroa mites by triggering the mite RNAi response. This symbiont-mediated RNAi approach is a tool for studying bee functional genomics and potentially for safeguarding bee health.
(Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
Databáze: MEDLINE
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