Induction of Mucosal Humoral Immunity by Subcutaneous Injection of an Oil-emulsion Vaccine against Salmonella enterica subsp. enterica serovar Enteritidis in Chickens.

Autor:	Ishida Y; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.; Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan., Sakai E; Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan., Sato K; Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan., Sugiyama E; Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan., Mima K; Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan., Taneno A; Choka Research Institute, vaxxinova Japan K.K., 809, Choka, Nikko-shi, Tochigi 321-1103, Japan., Shimomura H; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.; Department of Nutritional Science, Faculty of Human Life Science, Shokei University, 2-6-78, Kuhonji, Chuo-ku, Kumamoto-shi, Kumamoto 862-8678, Japan., Cui L; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan., Hirai Y; Division of Bacteriology, Department of Infection and Immunity, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.; Tamano Institute of Health and Human Services, 1-1-20 Chikko Tamano-shi, Okayama 706-0002, Japan.
Jazyk:	angličtina
Zdroj:	Food safety (Tokyo, Japan) [Food Saf (Tokyo)] 2018 Nov 20; Vol. 6 (4), pp. 151-155. Date of Electronic Publication: 2018 Nov 20 (Print Publication: 2018).
DOI:	10.14252/foodsafetyfscj.2018003
Abstrakt:	Salmonella enterica subsp. enterica serovar Enteritidis (SE) is one of the major causes of food poisoning. Much effort has been made to develop a vaccine for the prevention of SE colonization and infection in poultry. However, the effect of inactivated whole-cell SE vaccines on the bacterial attachment has not been clarified. This study investigated the immune responses to a killed whole-cell SE vaccine in chickens and the effect of vaccination on the bacterial attachment of SE to cultured Vero cells. A 1 ml dose of 10 8 -10 9 CFU viable SE bacterial cells was orally administered to chickens at 4 weeks or 10 months post vaccination. The number (CFU) of SE in 1 g of cecal droppings was counted on day 6 after administration. The SE CFUs were significantly lower ( p < 0.05) in the vaccinated chickens, not only at 4 weeks but also at 10 months after vaccination, than in the unvaccinated control chickens. Anti-SE IgG and anti-SE IgA were detected using enzyme-linked immunosorbent assay (ELISA) in serum and intestinal and oviduct fluid samples from vaccinated chickens. Adhesion of heat-killed SE cells to Vero cells was reduced by pre-treatment of the bacteria by the vaccinated chicken-derived intestinal fluid, indicating the potential of the vaccine-induced antibody to prevent SE adhesion to epithelial cell surfaces. (© Food Safety Commission, Cabinet Office, Government of Japan.)
Databáze:	MEDLINE
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