Characterization of Three Osmotin-Like Proteins from Plumeria rubra and Prospection for Adiponectin Peptidomimetics.

Autor:	Freitas CDT; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil., Nishi BC; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil., do Nascimento CTM; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil., Silva MZR; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil., Bezerra EHS; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil., Rocha BAM; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil., Grangeiro TB; Departamento de Biologia, Fortaleza, Universidade Federal de Ceara, Ceara, Brazil., Oliveira JPB; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil., Souza PFN; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil., Ramos MV; Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.
Jazyk:	angličtina
Zdroj:	Protein and peptide letters [Protein Pept Lett] 2020; Vol. 27 (7), pp. 593-603.
DOI:	10.2174/0929866527666200129154357
Abstrakt:	Background: Osmotin-Like Proteins (OLPs) have been purified and characterized from different plant tissues, including latex fluids. Besides its defensive role, tobacco osmotin seems to induce adiponectin-like physiological effects, acting as an agonist. However, molecular information about this agonistic effect on adiponectin receptors has been poorly exploited and other osmotins have not been investigated yet. Objective and Methods: The present study involved the characterization of three OLPs from Plumeria rubra latex and molecular docking studies to evaluate the interaction between them and adiponectin receptors (AdipoR1 and AdipoR2). Results: P. rubra Osmotin-Like Proteins (PrOLPs) exhibited molecular masses from 21 to 25 kDa and isoelectric points ranging from 4.4 to 7.7. The proteins have 16 cysteine residues, which are involved in eight disulfide bonds, conserved in the same positions as other plant OLPs. The threedimensional (3D) models exhibited the three typical domains of OLPs, and molecular docking analysis showed that two PrOLP peptides interacted with two adiponectin receptors similarly to tobacco osmotin peptide. Conclusion: As observed for tobacco osmotin, the latex osmotins of P. rubra exhibited compatible interactions with adiponectin receptors. Therefore, these plant defense proteins (without known counterparts in humans) are potential tools to study modulation of glucose metabolism in type II diabetes, where adiponectin plays a pivotal role in homeostasis. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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