Parenteral vaccination protects against transcervical infection with Chlamydia trachomatis and generate tissue-resident T cells post-challenge.

Autor:	Nguyen NDNT; 1Statens Serum Institut, Department for Infectious Disease Immunology, Copenhagen, Denmark., Olsen AW; 1Statens Serum Institut, Department for Infectious Disease Immunology, Copenhagen, Denmark., Lorenzen E; 1Statens Serum Institut, Department for Infectious Disease Immunology, Copenhagen, Denmark., Andersen P; 1Statens Serum Institut, Department for Infectious Disease Immunology, Copenhagen, Denmark., Hvid M; 2Department of Biomedicine and Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Follmann F; 1Statens Serum Institut, Department for Infectious Disease Immunology, Copenhagen, Denmark., Dietrich J; 1Statens Serum Institut, Department for Infectious Disease Immunology, Copenhagen, Denmark.
Jazyk:	angličtina
Zdroj:	NPJ vaccines [NPJ Vaccines] 2020 Jan 23; Vol. 5 (1), pp. 7. Date of Electronic Publication: 2020 Jan 23 (Print Publication: 2020).
DOI:	10.1038/s41541-020-0157-x
Abstrakt:	The optimal protective immunity against Chlamydia trachomatis ( C.t.) is still not fully resolved. One of the unresolved issues concerns the importance of resident immunity, since a recent study showed that optimal protection against a transcervical (TC) infection required genital tissue-resident memory T cells. An important question in the Chlamydia field is therefore if a parenteral vaccine strategy, inducing only circulating immunity primed at a nonmucosal site, should be pursued by Chlamydia vaccine developers. To address this question we studied the protective efficacy of a parenteral Chlamydia vaccine, formulated in the Th1/Th17 T cell-inducing adjuvant CAF01. We found that a parenteral vaccination induced significant protection against a TC infection and against development of chronic pathology. Protection correlated with rapid recruitment of Th1/Th17 T cells to the genital tract (GT), which efficiently prevented infection-driven generation of low quality Th1 or Th17 T cells, and instead maintained a pool of high quality multifunctional Th1/Th17 T cells in the GT throughout the infection. After clearance of the infection, a pool of these cells settled in the GT as tissue-resident Th1 and Th17 cells expressing CD69 but not CD103, CD49d, or CCR7, where they responded rapidly to a reinfection. These results show that a nonmucosal parenteral strategy inducing Th1 and Th17 T cells mediates protection against both infection with C.t . as well as development of chronic pathology, and lead to post-challenge protective tissue-resident memory immunity in the genital tract. Competing Interests: Competing interestsThe authors declare no competing interests. (© The Author(s) 2020.)
Databáze:	MEDLINE
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