Genomic DNA PCR analysis to assess xenograft development in mouse mammary gland.
| Autor: | Aujean E; Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France., Laubier J; Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France., Brun N; Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France., Finot L; PEGASE, INRAE, Agrocampus Ouest, Saint-Gilles 35590, France., Chanat E; PEGASE, INRAE, Agrocampus Ouest, Saint-Gilles 35590, France., Dessauge F; PEGASE, INRAE, Agrocampus Ouest, Saint-Gilles 35590, France., Hue-Beauvais C; Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France., Provost FL; Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350, Jouy-en-Josas, France. |
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| Jazyk: | angličtina |
| Zdroj: | BioTechniques [Biotechniques] 2020 Apr; Vol. 68 (4), pp. 219-222. Date of Electronic Publication: 2020 Jan 28. |
| DOI: | 10.2144/btn-2019-0125 |
| Abstrakt: | The mouse transplantation model remains the most relevant methodology to assess the functional capacities of mammary cells and is particularly appropriate for investigations regarding mammary stem cells, whatever the species studied. Following xenotransplantation in mice mammary fat pad, the development of the xenograft is commonly evaluated by immunohistology. Here, we present a simple and rapid method to control the species specificity of a xenograft based on genomic DNA PCR amplification. DNA is extracted from the fixed samples intended for histology, thus allowing the reuse of precious samples. Standard and digital droplet PCR (requiring low DNA quantities) methods have been used to make the present method suitable for the analysis of xenotransplanted samples. |
| Databáze: | MEDLINE |
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