Data describing the experimental design and quality control of RNA-Seq of human adipose-derived stem cells undergoing early adipogenesis and osteogenesis.

Autor:	Marcon BH; Laboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil., Spangenberg L; Unidad de Bioinformática, Institut Pasteur Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay., Bonilauri B; Laboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil., Robert AW; Laboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil., Angulski ABB; Laboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil., Cabo GC; Unidad de Bioinformática, Institut Pasteur Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay., Cofré AR; Laboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil., Bettes PSL; Cirurgia Plástica Dr. Paulo Bettes, Rua Francisco Rocha, 1312, Curitiba, PR, 80730-390, Brazil., Dallagiovanna B; Laboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil., Shigunov P; Laboratory of Basic Biology of Stem Cells (LABCET), Instituto Carlos Chagas - FIOCRUZ-PR, Curitiba, Paraná, 81350-010, Brazil.
Jazyk:	angličtina
Zdroj:	Data in brief [Data Brief] 2019 Dec 31; Vol. 28, pp. 105053. Date of Electronic Publication: 2019 Dec 31 (Print Publication: 2020).
DOI:	10.1016/j.dib.2019.105053
Abstrakt:	An important tool to study the regulation of gene expression is the sequencing and the analysis of different RNA fractions: total, ribosome-free, monosomal and polysomal. By comparing these different populations, it is possible to identity which genes are differentially expressed and to get information on how transcriptional and translational regulation modulates cellular function. Therefore, we used this strategy to analyze the regulation of gene expression of human adipose-derived stem cells during the triggering of the adipogenic and osteogenic differentiation. Here, we have focused on analyzing the differential expression of mRNAs during early adipogenic and osteogenic differentiation, and presented the detailed data concerning the experimental design, the RNA-Seq quality data, the raw data obtained and the RT-qPCR validation data. This information is important to confirm the accuracy of the data considering a future reuse of the data provided. Moreover, this study may be used as groundwork for future characterization of the transcriptome and the translatome regulation of different cell types. (© 2019 The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu