The Olfactory Bulb Provides a Radioresistant Niche for Glioblastoma Cells.

Autor:	Timme CR; Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland., Degorre-Kerbaul C; Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland., McAbee JH; Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland; Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom; Wake Forest University School of Medicine, Winston-Salem, North Carolina., Rath BH; Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland., Wu X; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc, Frederick, Maryland., Camphausen K; Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland., Tofilon PJ; Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland. Electronic address: philip.tofilon@nih.gov.
Jazyk:	angličtina
Zdroj:	International journal of radiation oncology, biology, physics [Int J Radiat Oncol Biol Phys] 2020 May 01; Vol. 107 (1), pp. 194-201. Date of Electronic Publication: 2020 Jan 25.
DOI:	10.1016/j.ijrobp.2020.01.007
Abstrakt:	Purpose: The various microenvironments that exist within the brain combined with the invasive nature of glioblastoma (GBM) creates the potential for a topographic influence on tumor cell radiosensitivity. The aim of this study was to determine whether specific brain microenvironments differentially influence tumor cell radioresponse. Methods and Materials: GBM stem-like cells were implanted into the right striatum of nude mice. To measure radiosensitivity, proliferation status of individual tumor cells was determined according to the incorporation of 5-chloro-2'-deoxyuridine delivered at 4, 12, and 20 days after brain irradiation. As an additional measure of radiosensitivity, the percentage of human cells in the right hemisphere and the olfactory bulb were defined using digital droplet polymerase chain reaction. Targeted gene expression profiling was accomplished using NanoString analysis. Results: Tumor cells were detected throughout the striatum, corpus callosum, and olfactory bulb. After an initial loss of proliferating tumor cells in the corpus callosum and striatum after irradiation, there was only a minor recovery by 20 days. In contrast, the proliferation of tumor cells located in the olfactory bulb began to recover at 4 days and returned to unirradiated levels by day 12 postirradiation. The percentage of human cells in the right hemisphere and the olfactory bulb after irradiation also suggested that the tumor cells in the olfactory bulb were relatively radioresistant. Gene expression profiling identified consistent differences between tumor cells residing in the olfactory bulb and those in the right hemisphere. Conclusions: These results suggest that the olfactory bulb provides a radioresistant niche for GBM cells. (Published by Elsevier Inc.)
Databáze:	MEDLINE
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