An optimized protocol for high yield expression and purification of an extremophilic protein.

Autor: Malash MN; Microbiology and Immunology Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October City, Giza, Egypt; Biology Department, School of Sciences and Engineering, American University in Cairo, New Cairo, Cairo, Egypt; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Menofia, Egypt., Hussein NA; Molecular Biology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Cairo, Egypt., Muawia S; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Menofia, Egypt., Nasr MI; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Menofia, Egypt., Siam R; Biology Department, School of Sciences and Engineering, American University in Cairo, New Cairo, Cairo, Egypt; University of Medicine and Health Sciences, St. Kitts, West Indies. Electronic address: rsiam@aucegypt.edu.
Jazyk: angličtina
Zdroj: Protein expression and purification [Protein Expr Purif] 2020 May; Vol. 169, pp. 105585. Date of Electronic Publication: 2020 Jan 25.
DOI: 10.1016/j.pep.2020.105585
Databáze: MEDLINE