Genetic variations and haplotypic diversity in the Myostatin gene of New Zealand cattle breeds.

Autor: Haruna IL; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand. Electronic address: ishaku.haruna@lincolnuni.ac.nz., Ekegbu UJ; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand., Ullah F; Department of Biotechnology, University of Malakand, Dir lower 18800, Chakdara, Pakistan., Amirpour-Najafabadi H; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand., Zhou H; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand., Hickford JGH; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand. Electronic address: Jonathan.hickford@lincoln.ac.nz.
Jazyk: angličtina
Zdroj: Gene [Gene] 2020 May 25; Vol. 740, pp. 144400. Date of Electronic Publication: 2020 Jan 24.
DOI: 10.1016/j.gene.2020.144400
Abstrakt: Myostatin (MSTN) is a circulating factor that is secreted by muscle cells, and that acts upon those cells to inhibit the proliferation of muscle fibres during pre-natal muscle growth. The Polymerase Chain Reaction (PCR) coupled with Single Strand Conformational Polymorphism (SSCP) analysis, was used to reveal variation in the bovine MSTN gene (MSTN) in 722 cattle from a variety of breeds farmed in New Zealand (NZ). These included Hereford, Angus, Charolais, Simmental, Red Poll, South Devon, Shorthorn, Murray Grey, cross-bred Holstein-Friesian × Jersey cattle, and other composite breeds of cattle. Sequence analysis of five regions of MSTN that encompassed coding and non-coding regions of the gene, revealed a total of twelve single-nucleotide substitutions (7 in intron 1 and 5 in a region spanning the intron 2 - exon 3 boundary), and a single nucleotide deletion. Of these 12 substitutions, five are reported here for the first time, whereas seven have been previously described. The deletion c.748-78del, was located in the intron 2 - exon 3 boundary region, and has been reported previously. No nucleotide variation was identified in exons 1, 2 and 3. A total of 18 extended haplotypes were resolved spanning two variable regions (intron 1 and the intron 2 - exon 3 boundary), some of which were common across the breeds, while others were peculiar to particular breeds. The genetic variations identified provide insight into the conserved and polymorphic nature of the coding and non-coding sequences of bovine MSTN respectively, and thus provides a baseline for further study into how variation in the gene might affect growth and carcass traits in NZ cattle.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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