Mechanistic insight into the effect of BT-benzo-29 on the Z-ring in Bacillus subtilis.

Autor: Kunal K; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India., Tiwari R; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India., Dhaked HPS; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India., Surolia A; Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India., Panda D; Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Jazyk: angličtina
Zdroj: IUBMB life [IUBMB Life] 2020 May; Vol. 72 (5), pp. 978-990. Date of Electronic Publication: 2020 Jan 26.
DOI: 10.1002/iub.2234
Abstrakt: The assembly and disassembly of FtsZ play an essential role in bacterial cell division. Using single-cell imaging, we report that short exposure to BT-benzo-29 inhibits Z-ring formation in live Bacillus subtilis cells. Fluorescence recovery after photobleaching of the Z-ring in live bacteria demonstrated that BT-benzo-29 strongly suppressed the assembly dynamics of FtsZ in the Z-ring. Furthermore, B. subtilis cells expressing V275A-FtsZ resisted the antibacterial activity of BT-benzo-29 providing evidence that BT-benzo-29 inhibits bacterial proliferation by targeting FtsZ. In addition, a brief (8 min) exposure of BT-benzo-29 destroyed the Z-ring without perturbing the localization of a late cell division protein, DivIVA, the nucleoid segregation, and membrane permeability. BT-benzo-29, when used in combination with vancomycin and polymyxin B (PMB), produced a much stronger inhibitory effect on Bacillus subtilis and Escherichia coli cells, respectively. The combination index of BT-benzo-29 with vancomycin and PMB was determined to be <1, suggesting that BT-benzo-29 exhibits synergistic inhibitory effects on bacterial proliferation when used along with these antibiotics.
(© 2020 International Union of Biochemistry and Molecular Biology.)
Databáze: MEDLINE
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