Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E-cadherin: Turbot against gilthead sea bream.

Autor: Ronza P; Departamento de Anatomía, Producción Animal y Ciencias Clínicas Veterinarias, Universidade de Santiago de Compostela, Lugo, Spain., Estensoro I; Fish Pathology Group, Instituto de Acuicultura Torre de la Sal, Castellón, Spain., Bermúdez R; Departamento de Anatomía, Producción Animal y Ciencias Clínicas Veterinarias, Universidade de Santiago de Compostela, Lugo, Spain.; Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, Spain., Losada AP; Departamento de Anatomía, Producción Animal y Ciencias Clínicas Veterinarias, Universidade de Santiago de Compostela, Lugo, Spain., Pérez-Cordón G; Fish Pathology Group, Instituto de Acuicultura Torre de la Sal, Castellón, Spain.; Cryptosporidium Reference Unit, Public Health Wales, Singleton Hospital, Swansea, UK., Pardo BG; Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.; Departamento de Zoología, Genética y Antropología Física, Universidade de Santiago de Compostela, Lugo, Spain., Sitjà-Bobadilla A; Fish Pathology Group, Instituto de Acuicultura Torre de la Sal, Castellón, Spain., Quiroga MI; Departamento de Anatomía, Producción Animal y Ciencias Clínicas Veterinarias, Universidade de Santiago de Compostela, Lugo, Spain.; Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
Jazyk: angličtina
Zdroj: Journal of fish diseases [J Fish Dis] 2020 Mar; Vol. 43 (3), pp. 337-346. Date of Electronic Publication: 2020 Jan 26.
DOI: 10.1111/jfd.13130
Abstrakt: Enteromyxoses are relevant diseases for turbot and gilthead sea bream aquaculture. The myxozoan parasites invade the intestinal mucosa, causing a cachectic syndrome associated with intestinal barrier alteration; nonetheless, their pathological impact is different. Turbot infected by Enteromyxum scophthalmi develop more severe intestinal lesions, reaching mortality rates of 100%, whereas in E. leei-infected gilthead sea bream, the disease progresses slowly, and mortality rates are lower. The mechanisms underlying the different pathogenesis are still unclear. We studied the distribution and expression changes of E-cadherin, a highly conserved protein of the adherens junctions, in the intestine of both species by immunohistochemistry and quantitative PCR, using the same immunohistochemical protocol and common primers. The regular immunostaining pattern observed in control fish turned into markedly irregular in parasitized turbot, showing an intense immunoreaction at the host-parasite interface. Nevertheless, E-cadherin gene expression was not significantly modulated in this species. On the contrary, no evident changes in the protein distribution were noticed in gilthead sea bream, whereas a significant gene downregulation occurred in advanced infection. The results contribute to the understanding of the different host-parasite interactions in enteromyxoses. Host and parasite cells appear to establish diverse relationships in these species, which could underlie the different pathological picture.
(© 2020 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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