Nanograss sensor for selective detection of Pseudomonas aeruginosa by pyocyanin identification in airway samples.

Autor: Alatraktchi FA; Department of Science and Environment, Roskilde University, Roskilde, Denmark. Electronic address: alzahraa@ruc.dk., Dimaki M; Department of Bioengineering and Biomedicine, Technical University of Denmark, Kgs, Lyngby, Denmark., Støvring N; Department of Bioengineering and Biomedicine, Technical University of Denmark, Kgs, Lyngby, Denmark., Johansen HK; Department of Clinical Microbiology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Molin S; Novo Nordisk Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark., Svendsen WE; Department of Bioengineering and Biomedicine, Technical University of Denmark, Kgs, Lyngby, Denmark.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2020 Mar 15; Vol. 593, pp. 113586. Date of Electronic Publication: 2020 Jan 22.
DOI: 10.1016/j.ab.2020.113586
Abstrakt: Pyocyanin is a virulence factor solely produced by the pathogen Pseudomonas aeruginosa. Pyocyanin is also a redox active molecule that can be directly detected by electrochemical sensing. A nanograss (NG) based sensor for sensitive quantification of pyocyanin in sputum samples from cystic fibrosis (CF) patients is presented here. The NG sensors were custom made in a cleanroom environment by etching nanograss topography on the electrode surface followed by depositing 200 nm gold. The NG sensors were utilized for amperometric quantification of pyocyanin in spiked hypertonic saline samples, resulting in a linear calibration curve with a R 2 value of 0.9901 and a limit of detection of 172 nM. The NG sensors were applied in a small pilot test on five airway samples from five CF patients. The NG sensor was capable of identifying P. aeruginosa in the airway samples in 60 s without any sample pretreatment.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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