| Autor: |
Onasanya GO; Department of Animal Science, Federal University Dutse, Dutse, Nigeria.; Deparment of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.; Mecheri Sheep Research Station, Pottaneri, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.; Biotechnology Center, Postgraduate Research Institute in Animal Sciences, Kattupakkum, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India., Msalya GM; Mecheri Sheep Research Station, Pottaneri, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India. msalya@sua.ac.tz.; Department of Animal, Aquaculture and Range Sciences (DAARS), Sokoine University of Agriculture (SUA), PO Box 3004, Morogoro, Tanzania. msalya@sua.ac.tz., Thiruvenkadan AK; Mecheri Sheep Research Station, Pottaneri, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India., Sreekumar C; Biotechnology Center, Postgraduate Research Institute in Animal Sciences, Kattupakkum, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India., Tirumurugaan GK; Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India., Sanni TM; Deparment of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Decampos JS; Department of Animal Production, University of Ilorin, Ilorin, Kwara State, Nigeria., Amusan SA; Department of Animal production, Federal College of Animal Health and Production Technology, Moor Plantation, Ibadan, Nigeria., Olowofeso O; Deparment of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Fafiolu AO; Department of Animal Nutrition, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Okpeku M; Department of Genetics, School of Life Sciences, University of KwaZulu-Natal, Westvile Campus, Durban, South Africa., Yakubu A; Department of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, Shabu-Lafia Campus, Lafia, Nigeria., Ikeobi CO; Deparment of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. |
| Abstrakt: |
Heat shock protein (HSP) 90 gene provides protection and adaptation to thermal assault and certain polymorphisms have been associated to heat tolerance in humans and animals. Single nucleotide polymorphisms (SNPs) of HSP 90 gene were used to evaluate the scientific basis of heat tolerance in four zebu breeds of Nigeria. The DNA was extracted from skin tissue of 90 adult bulls representing White Fulani (WF), Sokoto Gudali (SG), Red Bororo (RB), and Ambala (AM). The SNPs were determined in DNAs using PCR, sequencing, and visualization and bio-editing by chromatogram in SeqMan Ngen tool. Subsequently, respective genotypes were constructed and genotypic and allelic frequencies were computed. Also, body parameters related to heat stress (HS) including body temperature (BT), rectal temperature (RT), and respiratory rates (RR) were taken for each animal before biological sampling and heat tolerance coefficient (HTC) was calculated. We detected four SNPs distinct/specific for each breed as follows: change from thymine (T) to guanine (G) at position 116 (T116G) in RB, G to cytosine (C) at 220 (G220C) in SG, G to adenine (A) at two positions, 346 (G346A) and 390 (G390A) in AM and WF, respectively. Heterozygous SNPs showed significantly lower values (P < 0.0001) for BT, RT, RR, and HTC than homozygous genotypes at all positions. We hypothesize that animals with heterozygous SNPs in exon 3 of HSP 90 may be tolerant to HS. These SNPs can be used as bio-markers for screening large populations of cattle for tolerance to hot tropical conditions in Nigeria and other sub-humid places. |
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