Redox-coupled proton pumping drives carbon concentration in the photosynthetic complex I.

Autor: Schuller JM; Department of Structural Cell Biology, Max Planck Institute of Biochemistry, 82152, Martinsried, Germany. janschu@biochem.mpg.de., Saura P; Department of Biochemistry and Biophysics, Stockholm University, SE-106 91, Stockholm, Sweden.; Center of Integrated Protein Science at the Department of Chemistry, Technical University of Munich, Garching, Germany., Thiemann J; Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780, Bochum, Germany., Schuller SK; Department of Structural Cell Biology, Max Planck Institute of Biochemistry, 82152, Martinsried, Germany., Gamiz-Hernandez AP; Department of Biochemistry and Biophysics, Stockholm University, SE-106 91, Stockholm, Sweden.; Center of Integrated Protein Science at the Department of Chemistry, Technical University of Munich, Garching, Germany., Kurisu G; Institute for Protein Research, Osaka University, Suita, Osaka, 565-0871, Japan.; Department of Macromolecular Science, Graduate School of Science, Osaka University, Toyonaka, 560-0043, Japan., Nowaczyk MM; Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780, Bochum, Germany. marc.m.nowaczyk@rub.de., Kaila VRI; Department of Biochemistry and Biophysics, Stockholm University, SE-106 91, Stockholm, Sweden. ville.kaila@dbb.su.se.; Center of Integrated Protein Science at the Department of Chemistry, Technical University of Munich, Garching, Germany. ville.kaila@dbb.su.se.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2020 Jan 24; Vol. 11 (1), pp. 494. Date of Electronic Publication: 2020 Jan 24.
DOI: 10.1038/s41467-020-14347-4
Abstrakt: Photosynthetic organisms capture light energy to drive their energy metabolism, and employ the chemical reducing power to convert carbon dioxide (CO 2 ) into organic molecules. Photorespiration, however, significantly reduces the photosynthetic yields. To survive under low CO 2 concentrations, cyanobacteria evolved unique carbon-concentration mechanisms that enhance the efficiency of photosynthetic CO 2 fixation, for which the molecular principles have remained unknown. We show here how modular adaptations enabled the cyanobacterial photosynthetic complex I to concentrate CO 2 using a redox-driven proton-pumping machinery. Our cryo-electron microscopy structure at 3.2 Å resolution shows a catalytic carbonic anhydrase module that harbours a Zn 2+ active site, with connectivity to proton-pumping subunits that are activated by electron transfer from photosystem I. Our findings illustrate molecular principles in the photosynthetic complex I machinery that enabled cyanobacteria to survive in drastically changing CO 2 conditions.
Databáze: MEDLINE
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