Taxonomy, phylogeny and host-parasite interaction of two novel Myxobolus species infecting Brycon orthotaenia from the São Francisco River, Brazil.

Autor: Naldoni J; Department of Ecology and Evolutionary Biology, Federal University of São Paulo - UNIFESP. Rua Professor Arthur Riedel, 275, Jardim Eldorado, CEP, 09972-270 Diadema, SP, Brazil., Pereira JOL; Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, São Paulo University, Avenida Duque de Caxias Norte, 225, CEP, 13635-900 Pirassununga, SP, Brazil., Milanin T; Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, São Paulo University, Avenida Duque de Caxias Norte, 225, CEP, 13635-900 Pirassununga, SP, Brazil. Electronic address: tmilanin@gmail.com., Adriano EA; Department of Ecology and Evolutionary Biology, Federal University of São Paulo - UNIFESP. Rua Professor Arthur Riedel, 275, Jardim Eldorado, CEP, 09972-270 Diadema, SP, Brazil; Department of Animal Biology, Institute of Biology, University of Campinas, Caixa Postal 6109, CEP, 13083-970 Campinas, SP, Brazil., da Silva MRM; Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, São Paulo University, Avenida Duque de Caxias Norte, 225, CEP, 13635-900 Pirassununga, SP, Brazil., Maia AAM; Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, São Paulo University, Avenida Duque de Caxias Norte, 225, CEP, 13635-900 Pirassununga, SP, Brazil.
Jazyk: angličtina
Zdroj: Parasitology international [Parasitol Int] 2020 Jun; Vol. 76, pp. 102061. Date of Electronic Publication: 2020 Jan 22.
DOI: 10.1016/j.parint.2020.102061
Abstrakt: Two new Myxobolus species were described infecting Brycon orthotaenia from the São Francisco River, in the state of Minas Gerais, Brazil. From a total of 39 B. orthotaenia collected, two specimens (5.1%) exhibited infection of the ovary and 12 specimens (30.8%) displayed infection of the liver. The plasmodia of both Myxobolus species were white and spherical measuring around 1 mm in length. The plasmodium found in the ovary showed mature myxospores, which were oval shaped from the frontal view and measured 9.2-11.0 (9.8 ± 0.4) μm in length, 5.9-6.9 (6.5 ± 0.3) μm in width and 4.6-5 (4.9 ± 0.1) μm in diameter. The two polar capsules were the same size and measured 3.9-6.2 (4.7 ± 0.5) μm in length and 1.8-2.4 (2.1 ± 0.2) μm in width. The polar tubules had 9 coils. The plasmodium found in the liver showed mature myxospores which were ellipsoidal in shape from the frontal view and measured 10.0-11.4 (10.7 ± 0.5) μm in length, 7.3-8.6 (8.1 ± 0.4) μm in width and 5.3-7.0 (6.8 ± 0.4) μm in diameter. The two polar capsules were the same size and measured 4.2-5.4 (4.9 ± 0.3) μm in length and 1.9-2.9 (2.7 ± 0.3) μm in width. The polar tubules had 8 coils. Ultrastructural analysis revealed an asynchronous sporogenesis process, with young developmental myxospore stages more often found in the periphery of the plasmodium and mature myxospores in the centre of the plasmodium. The plasmodial wall was formed by a single membrane which was not surrounded by a layer of host tissue. A thick layer of fibrous material was found in the peripheral ectoplasm close to the plasmodial wall of the plasmodium found in the ovary. Phylogenetic analysis based on the small-subunit ribosomal DNA - ssrDNA sequences and using the closest myxozoan sequences to each one of the species studied here based on previous GenBank data and Henneguya/Myxobolus/Thelohanellus species parasitizing fish from South American, revealed that the new species are grouped in a subclade together with other Myxobolus species parasitizing bryconid hosts.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: