All-Optical Electrophysiology Reveals the Role of Lateral Inhibition in Sensory Processing in Cortical Layer 1.

Autor:	Fan LZ; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Kheifets S; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Böhm UL; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Wu H; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Piatkevich KD; Media Lab and McGovern Institute for Brain Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA., Xie ME; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Parot V; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Ha Y; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA., Evans KE; Harvard Medical School, Boston, MA, USA., Boyden ES; Media Lab and McGovern Institute for Brain Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA., Takesian AE; Harvard Medical School, Boston, MA, USA; Massachusetts Eye and Ear Infirmary, Boston, MA, USA., Cohen AE; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA; Department of Physics, Harvard University, Cambridge, MA, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USA. Electronic address: cohen@chemistry.harvard.edu.
Jazyk:	angličtina
Zdroj:	Cell [Cell] 2020 Feb 06; Vol. 180 (3), pp. 521-535.e18. Date of Electronic Publication: 2020 Jan 23.
DOI:	10.1016/j.cell.2020.01.001
Abstrakt:	Cortical layer 1 (L1) interneurons have been proposed as a hub for attentional modulation of underlying cortex, but the transformations that this circuit implements are not known. We combined genetically targeted voltage imaging with optogenetic activation and silencing to study the mechanisms underlying sensory processing in mouse barrel cortex L1. Whisker stimuli evoked precisely timed single spikes in L1 interneurons, followed by strong lateral inhibition. A mild aversive stimulus activated cholinergic inputs and evoked a bimodal distribution of spiking responses in L1. A simple conductance-based model that only contained lateral inhibition within L1 recapitulated the sensory responses and the winner-takes-all cholinergic responses, and the model correctly predicted that the network would function as a spatial and temporal high-pass filter for excitatory inputs. Our results demonstrate that all-optical electrophysiology can reveal basic principles of neural circuit function in vivo and suggest an intuitive picture for how L1 transforms sensory and modulatory inputs. VIDEO ABSTRACT. Competing Interests: Declaration of Interests A.E.C. is a founder of Q-State Biosciences and is on the scientific advisory board. A.E.C., L.Z.F., and S.K. have filed a patent on the imaging system. (Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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