Analysis of rumen contents and ocular fluid for toxic alkaloids from goats and cows dosed larkspur (Delphinium barbeyi), lupine (Lupinus leucophyllus), and death camas (Zigadenus paniculatus).

Autor: Lee ST; Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 1150 E. 1400 N., Logan, UT, 84341, USA. Electronic address: stephen.lee@ars.usda.gov., Welch KD; Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 1150 E. 1400 N., Logan, UT, 84341, USA., Stonecipher CA; Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 1150 E. 1400 N., Logan, UT, 84341, USA., Cook D; Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 1150 E. 1400 N., Logan, UT, 84341, USA., Gardner DR; Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 1150 E. 1400 N., Logan, UT, 84341, USA., Pfister JA; Poisonous Plant Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 1150 E. 1400 N., Logan, UT, 84341, USA.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2020 Mar; Vol. 176, pp. 21-29. Date of Electronic Publication: 2020 Jan 11.
DOI: 10.1016/j.toxicon.2020.01.003
Abstrakt: Larkspurs, lupines, and death camas can be acutely toxic to livestock and are serious poisonous plant problems in western North America. The toxicity of these plants depends on the composition and concentrations of the toxic alkaloids in the plants. In this study, goats and cows were dosed sub-lethal doses of larkspur, lupine, and death camas. Rumen contents and ocular fluid samples were collected, and simple extraction, sample preparation, and analytical methods were developed for the detection of toxic alkaloids in the rumen contents and ocular fluid samples. Toxic alkaloids were detected in the rumen contents and ocular fluid samples from the goats and cows dosed larkspur, lupine, and death camas. In addition, results from a case report where rumen contents were analyzed from a steer that was suspected to have died due to larkspur are reported. This demonstrates the utility of the methods described for the diagnosis of acute plant poisonings.
Competing Interests: Declaration of competing interest There is no conflict of interest for this work.
(Published by Elsevier Ltd.)
Databáze: MEDLINE
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