| Autor: |
Lee NY; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea., Yoon SJ; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea., Han DH; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea., Gupta H; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea., Youn GS; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea., Shin MJ; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea., Ham YL; Department of Nursing, Daewon University College , Jaecheon, Republic of Korea., Kwak MJ; ChunLab, Inc ., Seoul, Republic of Korea., Kim BY; ChunLab, Inc ., Seoul, Republic of Korea., Yu JS; Department of Bio and Fermentation Convergence Technology, BK21 PLUS Program, Kookmin University , Seoul, Republic of Korea.; Department of Agricultural Biotechnology, Seoul National University , Seoul, Republic of Korea., Lee DY; Department of Agricultural Biotechnology, Seoul National University , Seoul, Republic of Korea., Park TS; Department of Life Science, Gachon University , Sungnam, Republic of Korea., Park SH; Department of Life Science, Gachon University , Sungnam, Republic of Korea., Kim BK; Chong Kun Dang Bio Research Institute, Chong Kun Dang Bio Research Institute , Ansan-si, Gyeonggi-do, Republic of Korea., Joung HC; Chong Kun Dang Bio Research Institute, Chong Kun Dang Bio Research Institute , Ansan-si, Gyeonggi-do, Republic of Korea., Choi IS; Chong Kun Dang Bio Research Institute, Chong Kun Dang Bio Research Institute , Ansan-si, Gyeonggi-do, Republic of Korea., Hong JT; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea.; Department of Internal Medicine, Hallym University College of Medicine , Chuncheon, Republic of Korea., Kim DJ; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea.; Department of Internal Medicine, Hallym University College of Medicine , Chuncheon, Republic of Korea., Han SH; Department of Pathology, Hallym University College of Medicine , Chuncheon, Republic of Korea., Suk KT; Institute for Liver and Digestive Diseases, Hallym University , Chuncheon, Republic of Korea.; Department of Internal Medicine, Hallym University College of Medicine , Chuncheon, Republic of Korea. |
| Abstrakt: |
Targeting the gut-liver axis by modulating the gut-microbiome can be a promising therapeutic approach in nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the effects of single species and a combination of Lactobacillus and Pediococcus in NAFLD mice model. Six-week male C57BL/6J mice were divided into 9 groups (n = 10/group; normal, Western diet, and 7 Western diet-strains [10 9 CFU/g, 8 weeks]). The strains used were L. bulgaricus, L. casei, L. helveticus, P. pentosaceus KID7, and three combinations (1: L. casei+L. helveticus , 2: L. casei+L. helveticus+P. pentosaceus KID7, and 3: L. casei+L. helveticus+L. bulgaricus ). Liver/Body weight ratio, serum and stool analysis, liver pathology, and metagenomics by 16S rRNA-sequencing were examined. In the liver/body ratio, L. bulgaricus (5.1 ± 0.5), L. helveticus (5.2 ± 0.4), P. pentosaceus KID7 (5.5 ± 0.5), and combination1 and 2 (4.2 ± 0.6 and 4.8 ± 0.7) showed significant reductions compared with Western (6.2 ± 0.6)(p < 0.001). In terms of cholesterol and steatosis/inflammation/NAFLD activity, all groups except for L. casei were associated with an improvement ( p < .05). The elevated level of tumor necrosis factor-α/interleukin-1β (pg/ml) in Western (65.8 ± 7.9/163.8 ± 12.2) was found to be significantly reduced in L. bulgaricus (24.2 ± 1.0/58.9 ± 15.3), L. casei (35.6 ± 2.1/62.9 ± 6.0), L. helveticus (43.4 ± 3.2/53.6 ± 7.5), and P. pentosaceus KID7 (22.9 ± 3.4/59.7 ± 12.2)(p < 0.01). Cytokines were improved in the combination groups. In metagenomics, each strains revealed a different composition and elevated Firmicutes / Bacteroidetes ratio in the western (47.1) was decreased in L. bulgaricus (14.5), L. helveticus (3.0), and P. pentosaceus KID7 (13.3). L. bulgaricus, L. casei, L. helveticus , and P. pentosaceus KID7 supplementation can improve NAFLD-progression by modulating gut-microbiome and inflammatory pathway. |
