| Autor: |
Lew LC; School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia.; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800 Penang, Malaysia., Hor YY; School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia.; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800 Penang, Malaysia., Jaafar MH; School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia.; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800 Penang, Malaysia., Lau ASY; School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia., Ong JS; School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia., Chuah LO; School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia., Yap KP; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia., Azzam G; School of Biological Science, Universiti Sains Malaysia, 11800 Penang, Malaysia.; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800 Penang, Malaysia., Azlan A; School of Biological Science, Universiti Sains Malaysia, 11800 Penang, Malaysia., Liong MT; School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia.; USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, 11800 Penang, Malaysia. |
| Abstrakt: |
This study aimed to evaluate the anti-ageing effects of different strains of lactobacilli putative probiotics on an ageing rat model as induced by D-galactose and a high fat diet. Male Sprague-Dawley rats were fed with high fat diet (54% kcal fat) and injected with D-galactose daily for 12 weeks to induce ageing. The effects of putative probiotic strains on age-related impairment such as telomere length, plasma lipid peroxidation, hepatic 5'adenosine monophosphate-activated protein kinase (AMPK) expression, as well as endurance performance were evaluated. Administration of statin, Lactobacillus plantarum DR7 (LP-DR7), Lactobacillus fermentum DR9 (LF-DR9), and Lactobacillus reuteri 8513d (LR-8513d) significantly reduced the shortening of telomere and increased the expression of AMPK subunit-α1 ( P <0.05). Plasma lipid peroxidation was lower ( P <0.05) in groups administered with statin and LF-DR9 as compared to the control. AMPK subunit-α2 was elevated in rats administered with LP-DR7 as compared to the control ( P <0.05). Using an in vivo ageing rat model, the current study has illustrated the potentials of lactobacilli putative probiotics in alleviation of age-related impairment in a strain-dependent manner. |
