An Unexpected Case of Lagochilascariasis: Interdisciplinary Management and Use of 12S and 18S rDNA Analysis.

Autor:	Martinez-Hernandez F; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico., Prado-Calleros HM; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico., Ramirez-Hinojosa JP; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico., Figueroa-Angel V; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico., Lopez-Reynoso MT; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico., Jimenez-Andrade MDC; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico., Estrada-Moscoso I; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico., Rivas N; Laboratorio de Entomologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico., Escobedo-Ortegon J; Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi' Universidad Autonoma de Yucatan, Merida, Yucatan, Mexico., Flisser A; Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), Mexico City, Mexico., Romero-Valdovinos MG; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico. Electronic address: mirzagrv@yahoo.com., Maravilla P; Hospital General 'Dr. Manuel Gea Gonzalez', Mexico City, Mexico. Electronic address: maravillap@yahoo.com.
Jazyk:	angličtina
Zdroj:	The American journal of the medical sciences [Am J Med Sci] 2020 Apr; Vol. 359 (4), pp. 235-241. Date of Electronic Publication: 2020 Jan 07.
DOI:	10.1016/j.amjms.2020.01.001
Abstrakt:	A Mexican 24-year-old male patient was referred to our hospital due to increased left retroauricular volume with skin fistulisation, resembling an infection by the uncommon worm Lagochilascaris minor. The patient was submitted to lateral skull base surgery. No adult worms or eggs were observed during light and scanning electron microscopy analysis, as well as by histopathologic examination of the small piece of removed tissue, only L3 stage larvae of Lagochilascaris spp. were identified. Polymerase chain reaction-sequencing assays were performed using primers for the mitochondrial 12S and the nuclear 18S rDNA gene. DNA of some L minor adults, previously identified, were used as control. The molecular analysis identified the worm as L minor. According to previous reports, lagochilascariasis is a complicated infection that requires an interdisciplinary management by different clinical specialists. This is the first time that 12S and 18S rDNA genes are reported as molecular markers for diagnosis of L minor. (Copyright © 2020 Southern Society for Clinical Investigation. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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