| Autor: |
de Carvalho Silvello MA; Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, University of Campinas (UNICAMP), Monteiro Lobato n. 80, Cidade Universitária, Campinas, São Paulo, 13083-862, Brazil., Martínez J; Laboratory of High Pressure in Food Engineering, School of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Goldbeck R; Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, University of Campinas (UNICAMP), Monteiro Lobato n. 80, Cidade Universitária, Campinas, São Paulo, 13083-862, Brazil. goldbeck@unicamp.br. |
| Abstrakt: |
In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast® 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel®, 2% w.v -1 ) using a central composite rotatable design. Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm -2 ), and reaction temperature (25 to 50 °C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined. US intensity had a positive effect on enzyme activity. Under optimal conditions (170 s, 180.8 W cm -2 , and 25 °C), CMCase activity was 13% higher than that of the control. In the presence of substrate, CMCase activity increased by 33.87% and K M reduced by 23% in relation to that of the control. The theoretical yield of cellulose was 42.08%. Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application. |
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