Garlic passion fruit (Passiflora tenuifila Killip): Assessment of eventual acute toxicity, anxiolytic, sedative, and anticonvulsant effects using in vivo assays.

Autor: Holanda DKR; Federal University of Ceará, Department of Food Engineering, 60356-000 Fortaleza, Ceará, Brazil., Wurlitzer NJ; Embrapa Agroindústria Tropical, Dra Sara Mesquita, 2270, 60511-110 Fortaleza, Ceará, Brazil. Electronic address: nedio.jair@embrapa.br., Dionisio AP; Embrapa Agroindústria Tropical, Dra Sara Mesquita, 2270, 60511-110 Fortaleza, Ceará, Brazil., Campos AR; University of Fortaleza, Fortaleza, Ceará, Brazil., Moreira RA; University of Fortaleza, Fortaleza, Ceará, Brazil., Sousa PHM; Federal University of Ceará, Department of Food Engineering, 60356-000 Fortaleza, Ceará, Brazil., Brito ES; Embrapa Agroindústria Tropical, Dra Sara Mesquita, 2270, 60511-110 Fortaleza, Ceará, Brazil., Ribeiro PRV; Embrapa Agroindústria Tropical, Dra Sara Mesquita, 2270, 60511-110 Fortaleza, Ceará, Brazil., Iunes MF; Federal University of Ceará, Department of Food Engineering, 60356-000 Fortaleza, Ceará, Brazil., Costa AM; Embrapa Cerrados, Brasília, Distrito Federal, Brazil.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2020 Feb; Vol. 128, pp. 108813. Date of Electronic Publication: 2019 Nov 21.
DOI: 10.1016/j.foodres.2019.108813
Abstrakt: Several Passiflora species are known for their sedative and anxiolytic properties. However, the functional properties of Passiflora tenuifila Killip are still unexplored. The objective of this work was to evaluate the phenolic composition and acute toxicity, anxiolytic, sedative, and anticonvulsant effects using in vivo assays. The whole fruit (peel, pulp, and seed) was lyophilized and used for all assays. LC-MS showed 19 phenolic compounds, tentatively identified as flavonoids and phenolic acids. Acute treatment with single doses of up to 2000 mg kg -1 in Wistar rats showed no signs of mortality or toxicity over 14 days. The assay of functional effects was performed with Swiss mice, four groups, received by gavage, doses of P. tenuifila (200 or 400 mg kg -1 body weight), water, and diazepam (as negative and positive control), and behavior tests were performed after 60 min of the treatments. The animals treated with P. tenuifila fruit showed a significant decrease in locomotor activity, indicating a sedative and anxiolytic activity. No significant changes were observed in the rotarod apparatus, suggesting that the P. tenuifila fruit did not cause muscle relaxation. The 400 mg kg -1 dose of P. tenuifila exerted a protective effect against pentylenetetrazole-induced seizures, decreasing the severity and not causing the death of the animals. In conclusion, P. tenuifila showed no acute toxicity and had a promising effect as an anxiolytic agent, hypnotic-sedative and anticonvulsant, which could be related to its composition of flavonoids and phenolic acids.
(Copyright © 2019. Published by Elsevier Ltd.)
Databáze: MEDLINE
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