Different carbon sources result in differential activation of sigma B and stress resistance in Listeria monocytogenes.

Autor: Crespo Tapia N; Food Microbiology, Wageningen University & Research, the Netherlands., Dorey AL; Bacterial Stress Response Group, Microbiology, School of Natural Sciences, National University of Ireland, Galway, Galway H91 TK33, Ireland., Gahan CGM; School of Microbiology, University College Cork, Cork, Ireland; APC Microbiome Ireland, University College Cork, Cork, Ireland., den Besten HMW; Food Microbiology, Wageningen University & Research, the Netherlands., O'Byrne CP; Bacterial Stress Response Group, Microbiology, School of Natural Sciences, National University of Ireland, Galway, Galway H91 TK33, Ireland., Abee T; Food Microbiology, Wageningen University & Research, the Netherlands. Electronic address: tjakko.abee@wur.nl.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2020 May 02; Vol. 320, pp. 108504. Date of Electronic Publication: 2019 Dec 30.
DOI: 10.1016/j.ijfoodmicro.2019.108504
Abstrakt: Listeria monocytogenes is an important food-borne pathogen that is ubiquitous in the environment. It is able to utilize a variety of carbon sources, to produce biofilms on food-processing surfaces and to survive food preservation-associated stresses. In this study, we investigated the effect of three common carbon sources, namely glucose, glycerol and lactose, on growth and activation of the general stress response Sigma factor, SigB, and corresponding phenotypes including stress resistance. A fluorescent reporter coupled to the promoter of lmo2230, a highly SigB-dependent gene, was used to determine SigB activation via quantitative fluorescence spectroscopy. This approach, combined with Western blotting and fluorescence microscopy, showed the highest SigB activation in lactose grown cells and lowest in glucose grown cells. In line with this observation, lactose grown cells showed the highest resistance to lethal heat and acid stress, the highest biofilm formation, and had the highest adhesion/invasion capacity in Caco-2-derived C2Bbe1 cell lines. Our data suggest that lactose utilisation triggers a strong SigB dependent stress response and this may have implications for the resistance of L. monocytogenes along the food chain.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2019. Published by Elsevier B.V.)
Databáze: MEDLINE
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