Aptamer-Conjugated Superparamagnetic Ferroarabinogalactan Nanoparticles for Targeted Magnetodynamic Therapy of Cancer.

Autor: Kolovskaya OS; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science', 660036 Krasnoyarsk, Russia.; Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia., Zamay TN; Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia., Zamay GS; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science', 660036 Krasnoyarsk, Russia.; Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia., Babkin VA; Irkutsk Institute of Chemistry named after A.E. Favorsky, the Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, Russia., Medvedeva EN; Irkutsk Institute of Chemistry named after A.E. Favorsky, the Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, Russia., Neverova NA; Irkutsk Institute of Chemistry named after A.E. Favorsky, the Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, Russia., Kirichenko AK; Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia., Zamay SS; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science', 660036 Krasnoyarsk, Russia.; L.V. Kirensky Institute of Physics SB RAS-The Branch of Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', 660036 Krasnoyarsk, Russia., Lapin IN; Laboratory of Advanced Materials and Technology, Tomsk State University, 634050 Tomsk, Russia., Morozov EV; L.V. Kirensky Institute of Physics SB RAS-The Branch of Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', 660036 Krasnoyarsk, Russia.; Institute of Chemistry and Chemical Technology SB RAS-The Branch of Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', 660036 Krasnoyarsk, Russia., Sokolov AE; L.V. Kirensky Institute of Physics SB RAS-The Branch of Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', 660036 Krasnoyarsk, Russia.; School of Engineering Physics and Radio Electronics, Siberian Federal University, 660041 Krasnoyarsk, Russia., Narodov AA; Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia., Fedorov DG; Research Center for Computational Design of Advanced Functional Materials (CD-FMat), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan., Tomilin FN; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science', 660036 Krasnoyarsk, Russia.; L.V. Kirensky Institute of Physics SB RAS-The Branch of Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', 660036 Krasnoyarsk, Russia.; School of Non-Ferrous Metals and Materials Science, Siberian Federal University, 660041 Krasnoyarsk, Russia., Zabluda VN; L.V. Kirensky Institute of Physics SB RAS-The Branch of Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', 660036 Krasnoyarsk, Russia., Alekhina Y; Faculty of Physics, Department of Magnetism, Lomonosov Moscow State University, 119991 Moscow, Russia., Lukyanenko KA; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science', 660036 Krasnoyarsk, Russia.; Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia.; School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia., Glazyrin YE; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science', 660036 Krasnoyarsk, Russia.; Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia., Svetlichnyi VA; Laboratory of Advanced Materials and Technology, Tomsk State University, 634050 Tomsk, Russia., Berezovski MV; Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada., Kichkailo AS; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science', 660036 Krasnoyarsk, Russia.; Laboratory for Biomolecular and Medical Technologies, Faculty of Medicine, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, 660022 Krasnoyarsk, Russia.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2020 Jan 15; Vol. 12 (1). Date of Electronic Publication: 2020 Jan 15.
DOI: 10.3390/cancers12010216
Abstrakt: Nanotechnologies involving physical methods of tumor destruction using functional oligonucleotides are promising for targeted cancer therapy. Our study presents magnetodynamic therapy for selective elimination of tumor cells in vivo using DNA aptamer-functionalized magnetic nanoparticles exposed to a low frequency alternating magnetic field. We developed an enhanced targeting approach of cancer cells with aptamers and arabinogalactan. Aptamers to fibronectin (AS-14) and heat shock cognate 71 kDa protein (AS-42) facilitated the delivery of the nanoparticles to Ehrlich carcinoma cells, and arabinogalactan (AG) promoted internalization through asialoglycoprotein receptors. Specific delivery of the aptamer-modified FeAG nanoparticles to the tumor site was confirmed by magnetic resonance imaging (MRI). After the following treatment with a low frequency alternating magnetic field, AS-FeAG caused cancer cell death in vitro and tumor reduction in vivo. Histological analyses showed mechanical disruption of tumor tissues, total necrosis, cell lysis, and disruption of the extracellular matrix. The enhanced targeted magnetic theranostics with the aptamer conjugated superparamagnetic ferroarabinogalactans opens up a new venue for making biocompatible contrasting agents for MRI imaging and performing non-invasive anti-cancer therapies with a deep penetrated magnetic field.
Databáze: MEDLINE
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